Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, inhibition of CD45 functions4, and Western blotting3.
Application References:
1. Knapp, W., et al., 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Kishihara, K., et al., 1993. Cell 74:143. 3. Esser, M., et al., 2001. J. Virol. 75:6173. 4. Yamada, T., et al., 2002. J. Biol. Chem. 277:28830. 5. Nagano, M., et al., 2007. Blood 110:151.PubMed 6. Unpublished data. 7. Jiang, Q., et al. 2008. Blood.PubMed
Human peripheral blood lymphocytes stained with purified, followed by anti-mouse IgGs FITC
Description:
CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA), B220, and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
Other Names:
LCA, T200, B220
Structure:
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution:
Hematopoietic cells, not circulating erythrocytes or platelets
Function:
TCR and BCR mediated activation
Ligand Receptor:
Galectin-1, CD2, CD3, CD4
Antigen References:
1. Thomas, M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge, I., et al., 1994. Annu. Rev. Immunol.12:85.
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