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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, inhibition of CD45 functions4, and Western blotting3.
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Kishihara K, et al. 1993. Cell 74:143. 3. Esser M, et al. 2001. J. Virol. 75:6173. (WB) 4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. 5. Nagano M, et al. 2007. Blood 110:151. 6. Jiang Q, et al. 2008. Blood 112:2858.PubMed 7. Nagano M, et al. 2007. Blood 110:151. 8. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed 9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained with purified, followed by anti-mouse IgGs FITC
CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA), B220, and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
Other Names:
LCA, T200, B220
Structure:
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution:
Hematopoietic cells, not circulating erythrocytes or platelets
Function:
TCR and BCR mediated activation
Ligand Receptor:
Galectin-1, CD2, CD3, CD4
Antigen References:
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD45
Human peripheral blood lymphocytes stained with HI30 APC
Biotin anti-human CD45
Human peripheral blood lymphocytes stained with biotinylated HI30, followed by Sav-PE
FITC anti-human CD45
Human peripheral blood lymphocytes,
monocytes and granulocytes
stained with HI30 FITC
PE anti-human CD45
Human peripheral blood lymphocytes stained with HI30 PE
PE/Cy5 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 PE/CY5
Purified anti-human CD45
Human peripheral blood lymphocytes stained with purified, followed by anti-mouse IgGs FITC
APC/Cy7 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 APC/Cy7
PE/Cy7 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 PE/Cy7
Alexa Fluor® 488 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 Alexa Fluor® 647
Pacific Blue™ anti-human CD45
Human peripheral blood lymphocytes stained with HI30 Pacific Blue™
Alexa Fluor® 700 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 Alexa Fluor® 700
PerCP anti-human CD45
Human peripheral blood lymphocytes stained with HI30 PerCP
PerCP/Cy5.5 anti-human CD45
Human peripheral blood lymphocytes stained with HI30 PerCP/Cy5.5
Brilliant Violet 421™ anti-human CD45
Human peripheral blood lymphocytes were stained with CD45 (clone HI30) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-human CD45
RBC-lysed human peripheral blood cells were stained with CD45 (clone HI30) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
