Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemistry3,4,5 of acetone-fixed frozen sections, and blocking of T cell activation1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 300516).
Application References:
1. Knapp, W., et al., 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Moir, S., et al., 1999. J. Virol. 73:7972. 3. Deng, M. C., et al., 1995. Circulation 91:1647. 4. Friedman, T., et al., 1999. J. Immunol. 162:5256. 5. Mack, C.L., et al. 2004. Pediatr. Res. 56:79. 6. Lan, R.Y., et al. 2006. Hepatology 43:729.
Human peripheral blood lymphocytes stained with purified RPA-T4, followed by anti-mouse IgGs FITC
Description:
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation.
Other Names:
T4
Structure:
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution:
T cell subset, majority of thymocytes, monocytes/macrophages
Function:
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand Receptor:
MHC class II molecules, HIV gp120, IL-16
Antigen References:
1. Center, D., et al., 1996. Immunol. Today 17:476. 2. Gaubin, M., et al., 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
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