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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82. 2. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Human peripheral blood mononuclear cells stained with 561 FITC (lower panel) or FITC mouse IgG2a isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
CD34 is a type I monomeric sialomucin-like glycophophoprotein with the molecular weight about 105-120 kD, also know as gp105-120. It is selectively expressed on majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. The 561 antibody recognized epitope is in class III group which is resistant to sialidase/glycolyprotease and chymopapain treatment. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD34
Human peripheral blood mononuclear cells stained with 561 FITC (lower panel) or FITC mouse IgG2a isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
FITC anti-human CD34
Human peripheral blood mononuclear cells stained with CD34 (clone 561) FITC (top) or FITC mouse IgG2a isotype control (bottom) and CD45 (clone HI30) PerCP (gated on CD14- cell population).
PE anti-human CD34
Human peripheral blood mononuclear cells stained with 561 PE (lower panel) or PE mouse IgG2a isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
APC anti-human CD34
Human peripheral blood leukocytes stained with CD45 (HI30) FITC and 561 APC (top) or mIgG1,κ APC isotype control (bottom). Data was generated by gating on live CD14 negative lymphocyte population
