Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation, Westrn blotting3, induction of cytokine production3, and immunohistochemical staining of acetone-fixed frozen tissue sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 303410).
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 3. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.
CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
Other Names:
Siglec-3, gp67, p67
Structure:
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with CD33 (clone WM53) APC.
PE anti-human CD33
Human peripheral blood lymphocytes,
monocytes and granulocytes
stained with WM53 PE
PE/Cy5 anti-human CD33
Human peripheral blood lymphocytes,
monocytes and granulocytes
stained with WM53 PE
Purified anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.
LEAF™ Purified anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with LEAF™ purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.
PerCP/Cy5.5 anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clone WM53) PerCP/Cy5.5 (red histogram), or mouse IgG1, κ PerCP/Cy5.5 (green histogram).
Brilliant Violet 421™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
