Antibodies
Account Login LEGENDClub
Search 
 
Products Site Search
Advanced Search Shopping Cart
Home Company Products Custom Assays Pathways Support Contact

Purified Anti-human CD33 Antibody
Material Safety Data Sheet (MSDS) Purified Anti-human CD33 Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-human CD33 Antibody
303401 25 µg $45.00     
303402 100 µg $85.00     
Clone: WM53
Isotype: Mouse IgG1, κ
Workshop
Number:
IV M-505
Reactivity: Human, Cross-Reactivity*: Chimpanzee
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4 °C.
Application: FC - Quality tested
IP*, WB3*, IHC(Frozen)* - *This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation, Westrn blotting3, induction of cytokine production3, and immunohistochemical staining of acetone-fixed frozen tissue sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 303410).
Application
References:
1. Knapp, W., et al., 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Favaloro, E. et al., 1988. Br. J. Haematol. 69:163.
3. Garnache-Ottou, F., et al., 2005. Blood 105:1256.
Human peripheral blood lymphocytes, monocytes

Human peripheral blood lymphocytes, monocytes and granulocytes stained with purified WM53, followed by anti-mouse IgGs FITC


Description: CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
Other Names: Siglec-3, gp67, p67
Structure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
Function: Adhesion and lectin activity
Ligand Receptor: Sugar chains containing sialic acid
Antigen
References:
1. Favaloro, E., et al., 1988. Br. J. Haematol. 69:163.
2. Freeman, S., et al., 1995. Blood 85:2005.
Purified anti-human CD15HI98FC,IHC
Purified anti-human CD33HIM3-4FC
Purified anti-human CD14M5E2FC,IHC
Purified anti-human CD13WM15FC,IHC
Purified Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICC, IF, IHC, IP, WB
APC Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Biotin Goat anti-mouse IgG (minimal x-reactivity)Poly4053ELISA,FC, WB
FITC Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
PE Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Cell Staining Buffer (FBS)FC,ICC,ICFC
RBC Lysis Buffer (10X)FC,ICFC