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Purified anti-human CD33 Antibody
Purified anti-human CD33 Antibody
303402 100 µg $85.00     

Product Details

Clone: WM53
Isotype: Mouse IgG1, κ
Isotype Control:Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
IV M-505
Reactivity: Human, Cross-Reactivity: Chimpanzee
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4 °C.
Application:

FC - Quality tested
IP, WB, IHC - Reported in the literature
CyTOF® - Validated

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation, Westrn blotting3, induction of cytokine production3, and immunohistochemical staining of acetone-fixed frozen tissue sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 303410).

Application
References:

1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
3. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)

Human peripheral blood lymphocytes, monocytes,
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.


Compare all formats



Description:

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Other Names: Siglec-3, gp67, p67
Structure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
Function: Adhesion and lectin activity
Ligand Receptor: Sugar chains containing sialic acid
Antigen
References:

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

GeneID: 945
Latest Publications: View the latest CD33 articles on HighwirePress.com
UniProt: View information about CD33 on UniProt.org
Keywords: Purified anti-human CD33, WM53, Purified, Siglec-3, gp67, p67, Human, Cross-Reactivity: Chimpanzee, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD33
    Human peripheral blood lymphocytes, monocytes,

    Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with CD33 (clone WM53) APC.

  • PE anti-human CD33
    Human peripheral blood lymphocytes,
monocytes and

    Human peripheral blood lymphocytes, monocytes and granulocytes stained with WM53 PE

  • PE/Cy5 anti-human CD33
    Human peripheral blood lymphocytes,
monocytes and

    Human peripheral blood lymphocytes, monocytes and granulocytes stained with WM53 PE

  • Purified anti-human CD33
    Human peripheral blood lymphocytes, monocytes,

    Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.

  • LEAF™ Purified anti-human CD33
    Human peripheral blood lymphocytes, monocytes,

    Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with LEAF™ purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.

  • PerCP/Cy5.5 anti-human CD33
    Human peripheral blood monocytes were

    Human peripheral blood monocytes were stained with CD33 (clone WM53) PerCP/Cy5.5 (red histogram), or mouse IgG1, κ PerCP/Cy5.5 (green histogram).

  • Brilliant Violet 421™ anti-human CD33
    Human peripheral blood monocytes were

    Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

  • Brilliant Violet 570™ anti-human CD33
    Human peripheral blood monocytes were

    Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).

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