The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Human peripheral blood monocytes stained
with HIM3-4 FITC
Description:
CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
Other Names:
Siglec-3, gp67, p67
Structure:
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
