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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Nicoll G, et al. 1999. J. Biol Chem. 274:34089.
Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cy5.5 and purified S7.7 conjugated to PE
Human peripheral blood monocytes stained with purified S7.7 conjugated to PE
Siglec-7 is a 75 kD type I transmembrane protein and a member of the family of sialic acid-binding immunoglobulin-like lectins (Siglecs), also known as p75/AIRM1. It is primarily found on NK cells and monocytes. The cytoplasmic domain of Siglec-7 contains immunoreceptor tyrosine-based inhibitory motif (ITIM). CD328 mediates sialic acid-dependent cell-cell binding and functions as an inhibitory receptor of NK cells. CD328 preferentially binds to sialylated glycans with α2,8 disialyl and α2,6 sialyl residues.
Type I transmembrane protein, Siglec family member, 75 kD
Distribution:
Monocytes, NK cells
Function:
Inhibitory receptor of NK cells
Ligand Receptor:
Sialylated glycans (with α,8 disialyl and α2,6 sialyl residues)
Interaction:
After tyrosine phosphorylation interacts with PTPN6 and SHP-1
Bioactivity/Activities:
Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Antigen References:
1. Avril T, et al. 2006. Infect Immun. 74:4133. 2. Avril T, et al. 2006. J. Leukoc. Biol. 80:787. 3. Orr SJ, et al. 2007. J. Biol Chem. 282:3418. 4. Brunetta E, et al. 2009. Blood 114:3822.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD328 (Siglec-7)
Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cy5.5 and purified S7.7 conjugated to PE
Human peripheral blood monocytes stained with purified S7.7 conjugated to PE
PE anti-human CD328 (Siglec-7)
Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cy5.5 and S7.7 PE
Human peripheral blood monocytes stained with S7.7 PE
