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Purified anti-human CD301 (CLEC10A) Antibody
Purified anti-human CD301 (CLEC10A) Antibody
354701 25 µg $75.00 Qty:
    
354702 100 µg $175.00 Qty:
    

Product Details

 
Clone: H037G3 (See other available formats)
Isotype: Mouse IgG2a, κ
Isotype Control:Purified Mouse IgG2a, κ Isotype Ctrl
Reactivity: Human
Immunogen: Recombinant CD301 (extracellular domain)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Application
Notes:

H037G3 antibody binding is divalent cation dependent.

Purified anti-human CD301 (CLEC10A) Antibody H037G3 Image 1
Human monocyte-derived dendritic cells were stained with purified CD301 (clone H037G3) (filled histogram) or mouse IgG2a, κ isotype control, followed by anti-mouse IgG-PE (open histogram).


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Cell Surface Immunofluorescence Staining Protocol




Description:

CD301, also known as CLEC10A, macrophage galactose/N-acetylgalactosamine (GalNAc) specific lectin (MGL), DCASGPR, and HML, is a 40 kD type II transmembrane glycoprotein, which belongs to the C-type lectin family. Human CD301 consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region.  CD301 is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages. The expression level is upregulated by immunosuppressant dexamethasone. Human CD301 has an exclusive specificity for rare terminal GalNAc structures, which are revealed on the tumor-associated mucin MUC1 and CD45 (RA, RB, and RC but not RO isoforms). This interaction inhibits effector T cell activation and induces their apoptosis. CD301 also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity.

Other Names: Macrophage galactose/Nacetylgalactosamine (GalNAc) specific lectin, MGL, DCASGPR, HML CLECSF13, CLECSF14, HML2
Structure: 40 kD type II transmembrane glycoprotein, consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region
Distribution: Immature myeloid dendritic cells, activated macrophages
Function: Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, immune response
Ligand Receptor: Glycans containing terminal GalNAc moieties
Interaction: Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
Antigen
References:

1. Van Vliet SJ, et al. 2006. Nat. Immunol. 7:1200.
2. Higashi N, et al. 2002. J. Biol. Chem. 277:20686.
3. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892.
4. Valladeau J, et al. 2001. J. Immunol. 167:5767.
5. Suzuki N, et al. 1996. J. Immunol. 156:128.

GeneID: 10462
Latest Publications: View the latest CD301 articles on HighwirePress.com
UniProt: View information about CD301 on UniProt.org
Keywords: Purified anti-human CD301 (CLEC10A), H037G3, Purified, Macrophage galactose/Nacetylgalactosamine (GalNAc) specific lectin, MGL, DCASGPR, HML CLECSF13, CLECSF14, HML2, Human, Flow Cytometry, Immunology, Antibodies


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Related Products

DescriptionCloneApplications
Purified Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICC, IF, IHC, IP, WB
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Other Formats

DescriptionCloneApplications
APC anti-human CD301 (CLEC10A)H037G3FC
PE anti-human CD301 (CLEC10A)H037G3FC


Related Pathways

Pathway
Dendritic Cells


Related Categories

Category
Cell Biology > Cell Adhesion > CD301
Cell Biology > Signaling/Signaling Intermediates > CD301 (CLEC10A)
Immunology > Human Immunology > Adhesion Molecules > CD301 (CLEC10A)
Immunology > Human Immunology > Innate Immunity > CD301 (CLEC10A)
Immunology > Human Immunology > Monocyte/Macrophage > CD301 (CLEC10A)
Immunology > Human Immunology > Dendritic cells > CD301 (CLEC10A)
Immunology > Human Immunology > CD and Related Molecules > CD301 (CLEC10A) > H037G3

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD301 (CLEC10A)
    Human monocyte-derived dendritic cells were

    Human monocyte-derived dendritic cells were stained with purified CD301 (clone H037G3) (filled histogram) or mouse IgG2a, κ isotype control, followed by anti-mouse IgG-PE (open histogram).

  • PE anti-human CD301 (CLEC10A)
    Human monocyte-derived dendritic cells were

    Human monocyte-derived dendritic cells were stained with CD301 (clone H037G3) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).

  • APC anti-human CD301 (CLEC10A)
    Human monocyte-derived dendritic cells were

    Human monocyte-derived dendritic cells were stained with CD301 (clone H037G3) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).

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