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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
H037G3 antibody binding is divalent cation dependent.
Human monocyte-derived dendritic cells were stained with purified CD301 (clone H037G3) (filled histogram) or mouse IgG2a, κ isotype control, followed by anti-mouse IgG-PE (open histogram).
CD301, also known as CLEC10A, macrophage galactose/N-acetylgalactosamine (GalNAc) specific lectin (MGL), DCASGPR, and HML, is a 40 kD type II transmembrane glycoprotein, which belongs to the C-type lectin family. Human CD301 consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region. CD301 is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages. The expression level is upregulated by immunosuppressant dexamethasone. Human CD301 has an exclusive specificity for rare terminal GalNAc structures, which are revealed on the tumor-associated mucin MUC1 and CD45 (RA, RB, and RC but not RO isoforms). This interaction inhibits effector T cell activation and induces their apoptosis. CD301 also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity.
40 kD type II transmembrane glycoprotein, consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region
Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
Antigen References:
1. Van Vliet SJ, et al. 2006. Nat. Immunol. 7:1200. 2. Higashi N, et al. 2002. J. Biol. Chem. 277:20686. 3. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892. 4. Valladeau J, et al. 2001. J. Immunol. 167:5767. 5. Suzuki N, et al. 1996. J. Immunol. 156:128.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD301 (CLEC10A)
Human monocyte-derived dendritic cells were stained with purified CD301 (clone H037G3) (filled histogram) or mouse IgG2a, κ isotype control, followed by anti-mouse IgG-PE (open histogram).
PE anti-human CD301 (CLEC10A)
Human monocyte-derived dendritic cells were stained with CD301 (clone H037G3) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
APC anti-human CD301 (CLEC10A)
Human monocyte-derived dendritic cells were stained with CD301 (clone H037G3) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
