Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining4,6,7 of acetone-fixed frozen sections, immunoprecipitation1, and activation of T cells2,3,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 300414).
Application References:
1. Salmeron, A., et al., 1991. J. Immunol. 147:3047. 2. Graves, J., et al., 1991. J. Immunol. 146:2102. 3. Lafont, V., et al., 2000. J. Biol. Chem. 275:19282. 4. Ryschich, E., et al., 2003. Tissue Antigens 62:48. 5. Thompson, A.G., et al., 2004. J. Immunol. 173:1671. 6. Sakkas, L. I., et al., 1998. Clin. and Diag. Lab. Immunol. 5:430. 7. Mack, C.L., et al. 2004. Pediatr. Res. 56:79. 8. Thompson, A.G., et al. 2004. J. Immunol.173:1671. PubMed 9. Thakral, D., et al., 2008. J. Immunol. 180:7431. (FC) PubMed
Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC
Description:
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. The UCHT1 antibody is able to induce T cell activation.
Other Names:
T3, CD3ε
Structure:
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution:
Mature T and NK-T cells, thymocyte differentiation
Function:
Antigen recognition, signal transduction, T cell activation
Ligand Receptor:
Peptide antigen bound to MHC
Antigen References:
1. Barclay, N., et al., 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly, P., et al., 1981. Eur. J. Immunol. 11:329. 3. Lanier, L., et al., 1986. J. Immunol. 137:2501-2507.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
