The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4° C.
Application:
FC - Quality tested IP1*, IHC(Frozen)4,6,7* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining4,6,7 of acetone-fixed frozen sections, immunoprecipitation1, and activation of T cells2,3,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 300414).
Application References:
1. Salmeron, A., et al., 1991. J. Immunol. 147:3047. 2. Graves, J., et al., 1991. J. Immunol. 146:2102. 3. Lafont, V., et al., 2000. J. Biol. Chem. 275:19282. 4. Ryschich, E., et al., 2003. Tissue Antigens 62:48. 5. Thompson, A.G., et al., 2004. J. Immunol. 173:1671. 6. Sakkas, L. I., et al., 1998. Clin. and Diag. Lab. Immunol. 5:430. 7. Mack, C.L., et al. 2004. Pediatr. Res. 56:79. 8. Thompson, A.G., et al. 2004. J. Immunol.173:1671Pubmed
Human peripheral blood lymphocytes stained with purified UCHT1 and then detected with anti-mouse IgGs FITC
Description:
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. The UCHT1 antibody is able to induce T cell activation.
Other Names:
T3, CD3ε
Structure:
Ig superfamily, with the subunits of CD3γ, CD3δ, ? (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution:
Mature T and NK-T cells, thymocyte differentiation
Function:
Antigen recognition, signal transduction, T cell activation
Ligand Receptor:
Peptide antigen bound to MHC
Antigen References:
1. Barclay, N., et al., 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly, P., et al., 1981. Eur. J. Immunol. 11:329. 3. Lanier, L., et al., 1986. J. Immunol. 137:2501-2507.
