Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation4-7 of T lymphocytes. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 300314).
Application References:
1.Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp, W.1989. Leucocyte Typing IV. Oxford University Press, New York. 3. Barclay, N., et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. 4. Sedelies, K. A., et al., 2004. J. Biol. Chem. 279:26581. 5. Rivollier, A., et al., 2004. Blood 104:4029. 6. Scharschmidt, E., et al., 2004. Mol. Cell Biol. 24:3860. 7. Smeltz, R. B., 2007. J. Immunol. 178:4786. 8.Sheng, W. et al.2007.ELSEVIER.580:6819.PubMed
Human peripheral blood lymphocytes stained with purified HIT3a and detected with anti-mouse IgGs FITC
Description:
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. The HIT3a antibody is able to stimulate T cell activation.
Other Names:
T3, CD3ε
Structure:
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution:
Mature T and NK-T cells, thymocyte differentiation
Function:
Antigen recognition, signal transduction, T cell activation
Ligand Receptor:
Peptide antigen bound to MHC
Antigen References:
1. Barclay, N., et al., 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly, P., et al., 1981. Eur. J. Immunol. 11:329. 3. Lanier, L., et al., 1986. J. Immunol. 137:2501-2507.
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