Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining3,5 of acetone-fixed frozen tissue sections, and activation4,7,8 of integrin β1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 303010).
Human peripheral blood lymphocytes stained with purified TS2/16, followed by anti-mouse IgGs FITC
BT474 breast cancer cells were stained with anti-CD29 (clone TS2/16) followed by DyLight™ 649 Goat anti-mouse Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
CD29 is a 130 kD single chain type I glycoprotein, known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins which include CD29 bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.
Other Names:
Integrin β1 chain, VLA-β chain, gpIIa
Structure:
Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 APC
PE anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 PE
PE/Cy5 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 PE/Cy5
Purified anti-human CD29
Human peripheral blood lymphocytes stained with purified TS2/16, followed by anti-mouse IgGs FITC
BT474 breast cancer cells were stained with anti-CD29 (clone TS2/16) followed by DyLight™ 649 Goat anti-mouse Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
LEAF™ Purified anti-human CD29
Human peripheral blood lymphocytes stained with LEAF™ purified TS2/16, followed by anti-mouse IgGs FITC
BT474 breast cancer cells were stained with anti-CD29 (clone TS2/16) followed by DyLight™ 649 Goat anti-mouse Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
APC/Cy7 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 APC/Cy7
Alexa Fluor® 488 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 Alexa Fluor® 647
Alexa Fluor® 700 anti-human CD29
Human peripheral blood lymphocytes stained with TS2/16 Alexa Fluor® 700
