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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Human B7-H3 transfected P815 cells were stained with CD276 (clone MIH42) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
B7-H3, assigned as CD276, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human B7-H3 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain, a signature intracellular domain, and an additional isoform, known as 4Ig-B7-H3, containing nearly exact tandem duplication of the IgV-IgC domain and most likely caused by exon duplication. B7-H3 mRNA is broadly expressed in normal tissues whereas its protein expression is relatively rare. The expression of B7-H3 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages. It can be upregulated during the maturation from monocytes to DCs, or during the interaction between DCs and regulatory T cells. B7-H3 has been shown to be a co-stimulatory molecule that inhibits T-cell responses. Recently, B7-H3 has been identified to bind TLT-2 involved in the intracellular signaling pathway.
Other Names:
B7RP2
Structure:
Type I transmembrane protein with extracellular variable-type immunoglobulin domain and intracellular domain
Distribution:
Dendritic cells, macrophages, nature killer cells, and induced T cells
Function:
Involved in intracellular signaling pathway
Ligand Receptor:
Trem like transcript 2 protein (TLT-2)
Interaction:
Co-stimulator molecule inhibits T-cell response. Binds Trem like transcript 2 protein (TLT-2) to involve in intracellular signaling.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD276 (B7-H3)
Human B7-H3 transfected P815 cells were stained with CD276 (clone MIH42) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
PE anti-human CD276 (B7-H3)
Human B7-H3 transfected P815 cells were stained with CD276 (clone MIH42) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
