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Purified Anti-human CD271 (NGFR) Antibody
Material Safety Data Sheet (MSDS) Purified Anti-human CD271 NGFR Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-human CD271 (NGFR) Antibody
345101 25 µg $75.00     
345102 100 µg $120.00     
Clone: ME20.4
Isotype: Mouse IgG1, κ
Reactivity: Human
Cross-Reactivity: Monkey, Raccoon, Sheep, Cat, Dog, Pig, Rabbit
Immunogen: WM245 melanoma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4 °C.
Application: FC - Quality tested
Inhibition, IP, WB, IHC - These applications have been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications for the relevant formats include: immunoprecipitation, western blot, immunohistochemical staining of frozen or paraffin embedded tissue sections, blocking of NGF binding
Application
References:
1. Alonzo, H., et al. 1984. Proc. Natl. Acad. Sci. USA 81:6681
2. Cattoretti, G, et al. 1993. Blood. 81:1726
3. Kamke, M.R., et al. 2005. Hear Res. 206:89
Human neuroblastoma cell line SK-N-MC

Human neuroblastoma cell line SK-N-MC stained with ME20.4 FITC


Description:

CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, NGFR, is a type I transmembrane protein with a MW of 75 kDa, expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands, (NGF, BDNF, NTF3 and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis, and to regulate cell differentiation and neurogenesis.

Other Names: p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, NGFR, Nerve Growth Factor Receptor
Structure: Type I transmembranal protein with a MW of 75 kDa. The extracellular portion contains four TNFR-Cys repeats that form the NGF binding domain; the intracellular portion contains a death domain.
Distribution: Expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells, melanocytes
Function: Apoptosis, differentiation, neurogenesis
Ligand Receptor: Nerve growth factor (NGF), Brain-derived neurotrophic factor (BDNF), Neurotrophin 3 (NTF3) , Neurotrophin 4 (NTF4)
Interaction: TRAF2, TRAF4, TRAF6, PTPN13, RANBP9
Bioactivity/Activities: Apoptosis, differentiation, neurogenesis
Antigen
References:
1. Friedman, M.J. et al. 2009. J Biol Chem. 284:27944
2. Rock, J.R. et al. 2009. Proc Natl Acad Sci U S A. 106:12771
3. Kidd, S.K. et al. 2008. Brain Res.1195:113
4. Peters, E.M., et al. 2007. Am J Pathol. 171:1872
5. Jansen, P. et al. 2007. Nat Neurosci. 10:1449
Purified Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICC, IF, IHC, IP, WB
Cell Staining Buffer (FBS)Soln4202FC,ICC,ICFC
RBC Lysis Buffer (10X)Soln4203FC,ICFC