Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications for the relevant formats include: immunoprecipitation, western blot, immunohistochemical staining of frozen or paraffin embedded tissue sections, blocking of NGF binding
Application References:
1. Alonzo, H., et al. 1984. Proc. Natl. Acad. Sci. USA 81:6681 2. Cattoretti, G, et al. 1993. Blood. 81:1726 3. Kamke, M.R., et al. 2005. Hear Res. 206:89
Human neuroblastoma cell line SK-N-MC stained with ME20.4 FITC
Description:
CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, NGFR, is a type I transmembrane protein with a MW of 75 kDa, expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands, (NGF, BDNF, NTF3 and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis, and to regulate cell differentiation and neurogenesis.
Type I transmembranal protein with a MW of 75 kDa. The extracellular portion contains four TNFR-Cys repeats that form the NGF binding domain; the intracellular portion contains a death domain.
Distribution:
Expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells, melanocytes
1. Friedman, M.J. et al. 2009. J Biol Chem. 284:27944 2. Rock, J.R. et al. 2009. Proc Natl Acad Sci U S A. 106:12771 3. Kidd, S.K. et al. 2008. Brain Res.1195:113 4. Peters, E.M., et al. 2007. Am J Pathol. 171:1872 5. Jansen, P. et al. 2007. Nat Neurosci. 10:1449
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