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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemistry of acetone-fixed frozen sections, and in vitro induction of caspase-dependent cell death1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 314006).
Application References:
1. Nakayama M, et al. 2003. J. Immunol. 170:341. (Apop) 2. Felli N, et al. 2005. J. Immunol. 175:1464. (Apop)
Human Fn14 transfected cells stained with purified ITEM-1, followed by anti-mouse IgG FITC
TWEAK receptor is also known as fibroblast growth factor inducible 14 kD protein (Fn14) or CD266. It is a 14 kD transmembrane protein that is expressed on a variety of tissue types. This receptor binds TWEAK and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The TWEAK receptor is rapidly induced during liver regeneration and m play a role in hepatocyte growth. TWEAK receptor engagement can induce cell death and may play a role in inflammation. The ITEM-1 antibody is useful for flow cytometric analysis, induction of tumor cells apoptosis or necrotic cell death, enhancing endothelial cell proliferation, and immunohistochemistry on frozen tissues.
Other Names:
Fibroblast growth factor-inducible 14 kD protein (Fn14), TNFRS12a
Structure:
Transmembrane TNF superfamily receptor, contains no death domain, 14 kD
Distribution:
Widely expressed, high constitutive expression in heart placenta, lung, muscle, kidney, pancreas, and HUVEC
Function:
Binds to TWEAK (CD255), thought to play a role in cell death and inflammation
Ligand Receptor:
TWEAK (CD255); interacts with cytoplasmic proteins TRAF1, 2, and 3
Antigen References:
1. Feng SL, et al. 2000. Am. J. Path. 156:1253. 2. Meighan-Mantha RL, et al. 1999. J. Biol. Chem. 274:33166. 3. Nakayama M, et al. 2003. J. Immunol. 170:341. 4. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with purified ITEM-1, followed by anti-mouse IgG FITC
PE anti-human CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with ITEM-1 PE
LEAF™ Purified anti-human CD266 (Fn14, TWEAK R)
Human Fn14 transfected cells stained with LEAF™ purified ITEM-1, followed by anti-mouse IgG FITC
