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Each lot of this antibody is quality control tested by sandwich ELISA. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). ELISA Capture: The purified CARL-2 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated CARL-1 antibody (Cat. No. 308304) as the detecting antibody. Neutralization1: In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308403).
Application References:
1. Nakayama, M. 2000. J. Exp. Med. 192:1373.
Human TWEAK transfected cells stained with purified Carl-2, followed by biotinylated anti-mouse IgG and Sav-PE
TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, and apoptosis in a number of human cell lines. The CARL-2 antibody can neutralize the bioactivity of natural or recombinant TWEAK.
Other Names:
TNF-like weak inducer of apoptosis, Apo-3 ligand (Apo3L), Death receptor 3-L (DR3-L), TNFSF 12, CD255
Structure:
TNF superfamily; 17 kD (Mammalian)
Distribution:
Normal tissue and tumor cells at low levels
Function:
Promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, apoptosis
Regulation:
Type II integral membrane protein readily cleaved from cell surface
Cellular Sources:
Transcripts widely expressed and abundant in most tissues; protein efficiently secreted
Cellular Targets:
Macrophages, astrocytes, endothelial cells
Receptors:
Apo-3 (DR3, WSL-1, TRAMP, LARD); TWEAKR
Bioactivity/Activities:
Weak apoptotic induction; mediate CD4+ T-cell killing of macrophages; induces IL-6, IL-8, ICAM-1 in astrocytes; HUVEC proliferation
Antigen References:
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Chicheportiche, Y., et al. 1997. J. Biol. Chem. 272:32401. 3. Kaplan, M., et al. 2000. J. Immunol. 164:2897. 4. Saas, P., et al. 2000. Glia 32:102. 5. Wiley, S., et al. 2001. Immunity 15:837.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD255 (TWEAK)
Human TWEAK transfected cells stained with purified Carl-2, followed by biotinylated anti-mouse IgG and Sav-PE
