Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Kmieciak, M., et al., 2009. J. Transl. Med. 7:89. (FC) PubMed
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with purified BC96 and anti-mouse IgGs FITC
Description:
CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β(CD122) and common γ(CD132) chains to form the high affinity IL-2R complex.
Other Names:
Low affinity IL-2R, IL-2R α chain, Tac, p55
Structure:
Type I transmembrane glycoprotein, 55 kD
Distribution:
Activated T and B cells, monocytes/macrophages
Function:
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand Receptor:
IL-2
Antigen References:
1. Taniguchi, T., et al., 1993. Cell 73:5. 2. Waldmann, T. 1991. J. Biol. Chem. 266:2681.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
