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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.
Application References:
1. Valiante NM and Trinchieri G. 1993. J. Exp. Med. 178:1397. 2. Chuang SS, et al. 2000. Immunology. 100:378. 3. Hoffmann SC, et al. 2011. J. Immunol. 186:2757. PubMed
Human peripheral blood lymphocytes stained with purified C1.7, followed by anti-mouse IgG FITC
CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including majority of CD8+ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.
Other Names:
2B4
Structure:
38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution:
NK, T subset, monocytes, basophils, and eosinophils
Function:
Activation and inhibition of NK cell function
Ligand Receptor:
CD48
Antigen References:
1. Lee KM, et al. 2003. J. Immunol. 170:4881. 2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159. 3. Messmer B, et al. 2006. J. Immunol. 176:4646. 4. Boles KS, et al. 2001. Immunol. Rev. 181:234.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD244 (2B4)
Human peripheral blood lymphocytes stained with purified C1.7, followed by anti-mouse IgG FITC
FITC anti-human CD244 (2B4)
Human peripheral blood lymphocytes stained with CD8 (RPA-T8) APC and C1.7 FITC
PE anti-human CD244 (2B4)
Human peripheral blood lymphocytes stained with C1.7 PE
APC anti-human CD244 (2B4)
Human peripheral blood lymphocytes stained with C1.7 APC
PerCP/Cy5.5 anti-human CD244 (2B4)
Human peripheral lymphocytes were stained with CD8 FITC, and CD244 (clone C1.7) PerCP/Cy5.5 (top) or mouse IgG1, κ PerCP/Cy5.5 isotype control (bottom).
