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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3 and immunohistochemical staining of acetone-fixed frozen tissue sections.
Application References:
1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York. 2. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York. 3. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF) 4. Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906.
Human whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC
CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).
Other Names:
Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
Structure:
GPI-linked glycoprotein, 35-45 kD
Distribution:
B cells, granulocytes, epithelial cells
Function:
B cell proliferation and differentiation
Ligand Receptor:
CD62P (P-Selectin)
Antigen References:
1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD24
Human peripheral blood lymphocytes stained ML5 FITC
PE anti-human CD24
Human peripheral blood lymphocytes stained ML5 PE
Purified anti-human CD24
Human whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC
Alexa Fluor® 488 anti-human CD24
Human peripheral blood lymphocytes stained ML5 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD24
Human peripheral blood lymphocytes stained ML5 Alexa Fluor® 647
PerCP/Cy5.5 anti-human CD24
Human peripheral lymphocytes were stained with CD19 FITC and CD24 (clone ML5) PerCP/Cy5.5 (top) or mouse IgG2a isotype control PerCP/Cy5.5 (bottom).
APC anti-human CD24
Human peripheral blood lymphocytes stained with ML-5 APC
PE/Cy7 anti-human CD24
Human peripheral blood lymphocytes stained with ML-5 PE/Cy7
Brilliant Violet 421™ anti-human CD24
Human peripheral blood lymphocytes stained with CD19 APC and CD24 (clone ML5) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
