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Purified anti-human CD235ab Antibody
Purified anti-human CD235ab Antibody
306602 100 µg ¥17,000     

Product Details

Clone: HIR2
Isotype: Mouse IgG2b, κ
Isotype Control:Purified Mouse IgG2b, κ Isotype Ctrl
Workshop
Number:
VII 70299
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application:

FC - Quality tested
CyTOF® - Validated

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
References:

1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.

Human red blood cells stained
Human red blood cells stained with CD235a/b PE


Compare all formats



Description:

The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.

Other Names: Glycophorin A/B, GPA/GPB
Structure: Sialoglycoprotein, 20 kD
Distribution: Erythrocytes
Antigen
References:

1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.

GeneID: 2993
Latest Publications: View the latest CD235ab articles on HighwirePress.com
UniProt: View information about CD235ab on UniProt.org
Keywords: Purified anti-human CD235ab, HIR2, Purified, Glycophorin A/B, GPA/GPB, Human, Immunology, Antibodies
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Cell Staining BufferFC, ICC, ICFC
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD235ab
    Human red blood cells stained

    Human red blood cells stained with HIR2 APC

  • PE anti-human CD235ab
    Human red blood cells stained

    Human red blood cells stained with CD235a/b PE

  • PE/Cy5 anti-human CD235ab
    Human red blood cells stained

    Human red blood cells stained with HIR2 PE/Cy5

  • Purified anti-human CD235ab
    Human red blood cells stained

    Human red blood cells stained with CD235a/b PE

  • FITC anti-human CD235ab
    Human peripheral red blood cells

    Human peripheral red blood cells stained with HIR2 FITC

  • Pacific Blue™ anti-human CD235ab
    Human erythrocytes stained with HIR2

    Human erythrocytes stained with HIR2 Pacific Blue™

  • PerCP/Cy5.5 anti-human CD235ab
     Human red blood cells

    Human red blood cells were stained with anti-human CD235ab (clone HIR2) PerCP/Cy5.5 (filled histogram) or mouse IgG2b, κ PerCP/Cy5.5 isotype control (open histogram).

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