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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen tissue sections1, immunofluorescence2
Application References:
1. Matsuzaki H and Seon BK. 1987. Cancer Res. 47:4283. 2. Seon BK, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:845.
Human T-lymphoblastic leukemia cell line Jurkat stained with purified SN1a (M3-3D9), followed by anti-mouse IgG FITC
CD231 is 150 kD (28-45 kD reduced) cell surface glycoprotein belonging to the Transmembrane 4 Superfamily (TM4SF also known as tetraspanins). It was originally described as T-cell Acute Lymphoblastic Leukemia Antigen (TALLA or TALLA-1). CD231 is expressed on T-cell acute lymphoblastic leukemia, neuroblastoma cells, skeletal muscle, spleen and normal brain neuron. CD231 plays a role in integrin-mediated migration, however, its biological function is not fully clear yet.
T-cell acute lymphoblastic leukemia, skeletal muscle, spleen, neuroblastoma cells and normal brain neuron
Antigen References:
1. Matsuzaki H and Seon BK. 1987. Cancer Res. 47:4283. 2. Seon BK, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:845. 3. Hara H and Seon BK. 1986. Proc. Natl. Acad. Sci. USA 84:3390.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human CD231 (TALLA)
Human T lymphoblastic leukemia cell line, Jurkat, stained with SN1a (M3-3D9) PE
Purified anti-human CD231 (TALLA)
Human T-lymphoblastic leukemia cell line Jurkat stained with purified SN1a (M3-3D9), followed by anti-mouse IgG FITC
