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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Human peripheral blood monocytes were stained with purified CD220 (clone B6.220) (filled histogram) or mouse IgG2b, κ isotype control, followed by biotinylated anti-mouse IgG and Sav-PE (open histogram).
CD220, also known as insulin receptor, is a type I transmembrane receptor tyrosine kinase composed of two extracellular α-subunits and two transmembrane β-subunits. Binding insulin, the insulin receptor forms a heterotetramer of two units to induce autophosphorylation and activation of the tyrosine kinase activity of the receptor. Activation of insulin receptor leads to subsequent downstream signaling in metabolic regulation, inducing glucose uptake, cell growth, differentiation, and apoptosis. Gene mutation in the insulin receptor or decreased insulin receptor signaling leads to insulin-resistant diabetes mellitus and noninsulin-dependent diabetes mellitus (diabetes mellitus type 2). Most normal cells constitutively express insulin receptors. In hematopoietic cells, insulin receptor is constitutively expressed on monocytes and selectively expressed on activated lymphocytes.
Other Names:
Insulin Receptor
Structure:
Consist of two alpha subunits and two beta subunits; the beta subunit passes through the cellular membrane and is linked by disulfide bonds.
Distribution:
Hematopoietic and non hematopoietic cells.
Function:
Regulates cell metabolism and growth.
Ligand Receptor:
Insulin
Interaction:
Ectonucleotide pyrophosphatase/phosphodiesterase, PTPN11, GRB10, GRB7, PRKCD, IRS1, SH2B1 and Mothers against decapentaplegic homolog 2.
Antigen References:
1. Viardot A, et al. 2006. Endocrinology 148:346. 2. Ward CW, et al. 2009. Bioessays 31:422. 3. Brindle NP, et al. 1990. Biochem. J. 268:615.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD220
Human peripheral blood monocytes were stained with purified CD220 (clone B6.220) (filled histogram) or mouse IgG2b, κ isotype control, followed by biotinylated anti-mouse IgG and Sav-PE (open histogram).
PE anti-human CD220
Human peripheral blood monocytes were stained with CD220 (clone B6.220) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
