Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking1-3 of hIL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308806).
Application References:
1. Liu, Y., et al., 1997. J. Immunol. 158:604. 2. Levings, M.K., et al., 2005. Blood 105:1162. 3. Goodier, M.R., et al., 2000. J. Immunol. 165:139. 4. Huang, YH., et al. 2009. J. Leukoc Biol. 86:273. PubMed
Whole blood lymphocytes stained
with purified 3F9, then detected
with biotinylated anti-rat IgG
followed by Sav-PE
Description:
CD210, also known as the IL-10 receptor, is a 90-110 kD protein expressed on T cells, B cells, NK cells, monocytes and macrophages. CD210 belongs to the class II cytokine receptor family which includes the IFN-γ receptor (CDw119), the IFN-α/β receptor (CD118) and tissue factor (CD142). The IL-10 receptor is involved in signal transduction by inducing phosphorylation of STAT1a and STAT3 and by inducing activation of Jak1 and Tyk.
Other Names:
IL-10 Receptor, IL-10R
Structure:
Ig superfamily, class II cytokine receptor family, transmembrane glycoprotein, 90-110 kD
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