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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Bühring HJ, et al. 1999. Blood 94:2343. 2. Bühring HJ, et al. 2001. Blood 97:3303. 3. Platz IJ, et al. 2001. Int. Arch. Allergy Immunol. 126:335. 4. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 5. Gernez Y, et al. 2011. Int. Arch Allergy Imm. 154:318. (FC) PubMed
Human basophilic leukemia cell line KU-812 stained with purified NP4D6, followed by anti-mouse IgGs FITC
CD203c is a transmembrane protein and a member of the ectoenzyme family involved in the hydrolysis of extracellular oligonucleotides, nucleoside phosphates and NAD (possesses ATPase and ATP pyrophosphatase activity). The molecular weight of CD203c was observed to be 130 and 150 kD under reducing conditions and 270 kD under non-reducing conditions. CD203c is expressed on basophils and mast cells and is highly expressed on activated basophils. Secretory glands in endometrium and glioma cells are also positive. CD203c is a multifunctional ectoenzyme involved in the clearance of extracellular nucleotides whose substrates include nucleoside triphosphates, nucleoside diphosphates, cAMP, and NAD.
Other Names:
E-NPP3, ENPP3
Structure:
Transmembrane ectoenzyme family involved in the hydrolysis of extracellular oligonucleotides, nucleoside phosphates and NAD (possesses ATPase and ATP pyrophosphatase activity); reduced molecular weight is 130 and 150 kD, unreduced is 270 kD
Distribution:
Basophils and mast cells; highly expressed on activated basophils. Secretory glands in endometrium and glioma cells are also positive.
Function:
Multifunctional ectoenzyme involved in the clearance of extracellular nucleotides
Ligand Receptor:
Substrates include nucleoside triphosphates, nucleoside diphosphates, cAMP, and NAD
Antigen References:
1. Yano Y, et al. 2003. Int. J. Mol. Med. 12:763. 2. Andoh K, et al. 1999. Biochim. Biophys. Acta 1446:213.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD203c (E-NPP3)
Human basophilic leukemia cell line KU-812 stained with purified NP4D6, followed by anti-mouse IgGs FITC
Biotin anti-human CD203c (E-NPP3)
Overnight cultured human PBMCs stained with PE anti-human IgE (MHE-1) and
biotinylated anti-human CD203c (NP4D6), followed by Sav-APC (gated on lymphocyte population based on SSC vs. FSC)
PE anti-human CD203c (E-NPP3)
Human basophilic leukemia cell line KU-812 stained with NP4D6 PE
APC anti-human CD203c (E-NPP3)
Overnight cultured human peripheral blood mononuclear cells stained with NP4D6 APC and CCR3 PE
PerCP/Cy5.5 anti-human CD203c (E-NPP3)
Overnight cultured human peripheral blood mononuclear cells stained with NP4D6 PerCP/Cy5.5 and CCR3 Alexa Fluor® 647
Brilliant Violet 421™ anti-human CD203c (E-NPP3)
Human peripheral blood lymphocytes were stained with CD193 APC and CD203c (clone NP4D6) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
