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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and blocking of T cell activation2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 300212).
Application References:
1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Aversa G, et al. 1987. Transplant. Proc. 19:277. (Block) 3. Zaretsky AG, et al. 2009. J. Exp Med. 206:991. (IHC) PubMed 4. Perona-Wright G, et al. 2010. Nat. Immunol. 11:520. (FC) PubMed 5. Thummler K, et al. 2010. J. Leukoc. Biol. 88:1041. 6. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC) 7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes stained with purified RPA-2.10, and detected with anti-mouse IgGs FITC
CD2 is a 50 kD type I transmembrane glycoprotein also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R). This immunoglobulin superfamily member is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion.
Other Names:
LFA-2, T11, SRBC-R
Structure:
Ig superfamily, type I transmembrane glycoprotein, 50 kD
Distribution:
T cells, NK cells, thymocytes, and thymic B cell subsets
Function:
T cell activation, adhesion
Ligand Receptor:
CD58 (LFA-3), CD48, CD59, CD15
Antigen References:
1. Bell G, et al. 1995. J. Immunol. 155:2805. 2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579. 3. Moingeon P, et al. 1989. Immunol. Rev. 111:111.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD2
Human peripheral blood lymphocytes stained with biotinylated RPA-2.10 and detected with Sav-PE
FITC anti-human CD2
Human peripheral blood lymphocytes stained with RPA-2.10 FITC
LEAF™ Purified anti-human CD2
Human peripheral blood lymphocytes stained with LEAFTM purified RPA-2.10, and detected with anti-mouse IgGs FITC
PE anti-human CD2
Human peripheral blood lymphocytes stained
with RPA-2.10 PE
PE/Cy5 anti-human CD2
Human peripheral blood lymphocytes stained with RPA-2.10 PE/Cy5
Purified anti-human CD2
Human peripheral blood lymphocytes stained with purified RPA-2.10, and detected with anti-mouse IgGs FITC
APC anti-human CD2
Human peripheral blood lymphocytes stained with CD2 (clone RPA-2.10) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
