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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Hayes SM, et al. 2001. J. Immunol. 166:403. (IF) 2. Martin LH, et al. 1987. P. Natl. Acad. Sci. USA 84:918993. (IF)
Human T-lymphoblastic leukemia cell line Molt-4 stained with purified SN13, followed by anti-mouse IgG FITC
CD1b is a 43 kD member of the immunoglobulin superfamily also known as R1. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. In humans, CD1 family consists of group I proteins (CD1a, CD1b, CD1c), group II (CD1d), and group III (CD1e). CD1b plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1b has been implicated in thymic T cell development.
Other Names:
R1
Structure:
immunoglobulin superfamily
Distribution:
cortical thymocytes, Langerhans cells, and dendritic cells
Antigen References:
1. Porcelli S, et al. 1992. Nature 360:5937. 2. Giuliani A, et al. 2001. Infect. Immun. 69:746170.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD1b
Human T-lymphoblastic leukemia cell line Molt-4 stained with purified SN13, followed by anti-mouse IgG FITC
FITC anti-human CD1b
Human T lymphoblastic leukemia cell line, Molt-4, stained with SN13 (K5-1B8) FITC
PE anti-human CD1b
Human T lymphoblastic leukemia cell line, MOLT-4, stained with SN13 PE
