Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: The 5E8 antibody is useful for immunofluorescent staining and flow cytometric analysis of CCR3 expression.
CC-chemokine receptor 3 (CCR3), also known as CC CKR3, MIP1-alpha receptor like-2, eotaxin receptor, and CD193, is a member of the G protein-coupled seven transmembrane receptors family. It binds to the CC chemokines eotaxin, eotaxin-2, and eotaxin-3 with high affinity. CCR3 has also been reported to bind RANTES, MCP-3, and MCP-4 with low affinity. CCR3 receptor is expressed on human eosinophils, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitor cells, Th2-like lymphocytes, and keratinocytes. CCR3 is thought to play a role in allergic diseases such as bronchial asthma and allergic rhinitis. CCR3 is a co-receptor for HIV-1 and HIV-2, and the binding of eotaxin with CCR3 has been shown to inhibit HIV infection in some cell types.
Other Names:
CC CKR3, MIP1-alpha receptor like-2, eotaxin receptor, CD193
Upregulated by high affinity Fc IgE receptor ligation (mast cells), RANTES (keratinocytes), IFNg (monocytes, neutrophils), HIV tat protein (basophils), IL-3, IL-5 and GM-CSF (CD34+ progenitor cells), IL-2 and IL-4(T cells). Downregulated by IL-3 and IL-1.
Receptors:
Eotaxin, eotaxin-2, eotaxin-3
Antigen References:
1. Gerard W, et al. 1996. J. Exp. Med. 183:2437. 2. Uguccioni C, et al. 1997. J. Clin. Invest. 100:1137. 3. Sallusto F, et al. 1997. Science 277:2005. 4. Loetscher P, et al.2001.J. Biol. Chem. 276:2986.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD193 (CCR3)
Human peripheral blood granulocytes stained with 5E8 PE and CD16 FITC
PE anti-human CD193 (CCR3)
Human peripheral blood granulocytes stained with 5E8 PE and CD16 FITC
Alexa Fluor® 647 anti-human CD193 (CCR3)
Human peripheral blood granulocytes stained with CD16 FITC and 5E8/CCR3 Alexa Fluor® 647
APC/Cy7 anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 FITC and CCR3 (clone 5E8) APC/Cy7 (top) or mouse IgG2b APC/Cy7 isotype control (bottom). Data shown was generated from granulocyte population gating.
Brilliant Violet 421™ anti-human CD193 (CCR3)
Human peripheral blood leukocytes were stained with CD16 FITC and CD193/CCR3 (clone 5E8) Brilliant Violet 421™ (top) or mouse IgG2b Brilliant Violet 421™ isotype control (bottom). Data shown was generated from granulocyte population gating.
