Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 302112).
Application References:
1. Schlossman, S., et al., 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Kishimoto, T., et al., 1997. Leucocyte Typing VI. Garland Press. London. 3. Van Epps, D.E., et al., 1989. J. Immunol. 143:3207. 4. Meerschaert, J., et al., 1994. J. Immunol. 152:1915. 5. Sithu, S.D., et al., 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. 6. Nakayama, H., et al., 2008. J. Leukocyte Biol. 83:728. PubMed 7. Carmona, G., et al., 2008. Blood 111:2640. PubMed 8. Tran, DQ., et al. 2009. J. Immunol. 182:2929. PubMed
Human peripheral blood lymphocytes stained with purified TS1/18, followed by anti-mouse IgGs FITC
Description:
CD18 is a 90-95 kD type I transmembrane protein also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
