The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C.
Application:
FC-Quality Tested IP* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported (for the relevant formats) applications include:immunoprecipitation.
Application References:
1. Matsuyama, W., et al., 2005 J. Immunol. 174:6490. 2. Vogel, W., et al., 2002. Haematologica 88:126.
Human DDR1 transfected cells stained with purified 51D6, followed by anti-mouse IgM PE
Description:
The 51D6 monclonal antibody recognizes human CD167a also known as discoidin domain receptor, DDR1, neuroepithelial tyrosine kinase, epithelial-specific receptor kinase, tyrosine kinase receptor E (trkE), cell adhesion kinase (CAK), PTK3, and RTK6. CD167a is a membrane type II receptor kinase, containing a factor VIII-like domain. Three isoforms of CD167a have been reported. Predicted molecular weights are: isoform 1, approximately 101.5 kD, isoform 2, approximately 101 kD, and isoform 3 approximately 97 kD. CD167a is expressed on epithelial cells, keratinocytes, leukocytes, monocytes and has been reported to be overexpressed in some breast carcinomas. CD167a expression can be upregulated by p53. CD167a has been reported to interact with a variety of proteins including collagen type II alpha 1, collagen type III alpha 1, collagen type V alpha 2, collagen type XI alpha 1, phospholipase gamma 1, SHC, and the lipid-anchored docking protein FRS2. CD167a can be phosphorylated on Y513. Animal studies suggest that CD167a is involved in arterial wound healing. The 51D6 antibody has been shown to be useful for flow cytometric detection of human CD167a as well as immunoprecipitation.
Membrane Type II receptor kinase, containing a factor VIII-like domain. Three isoforms have been reported. Predicted molecular weights are: isoform 1, approximately 101.5 kD, isoform 2, approximately 101 kD, and isoform 3 approximately 97 kD.
Distribution:
Epithelial cells, keratinocytes, leukocytes, monocytes. Overexpressed in some breast carcinomas. Expression can be upregulated by p53.
Function:
Receptor membrane tyrosine kinase. May be involved in arterial wound repair
Modification:
Phosphorylation (Y513)
Interaction:
CD167a has been reported to interact with a variety of proteins including collagen type II alpha 1, collagen type III alpha 1, collagen type V alpha 2, collagen type XI alpha 1, phospholipase gamma 1, SHC, lipid-anchored docking protein FRS2
Antigen References:
1. DiMarco, E., et al. 1993. J. Biol. Chem. 268:24290. 2. Hou, G., et al. 2001. J. Clin. Invest. 107:727. 3. Johnson, J.D., et al. 1993. Proc. Natl. Acad. Sci. USA 90:5677. 4. Sakuma, S., et al. 1996. FEBS Lett. 398:165.
