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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional reported applications include: block binding of PVR ligands CD226 and CD96 to PVR, immunoprecipitation, western blot
CD155, known as poliovirus receptor (PVR) or nectin-like 5, is a 70 kD type I transmembrane glycoprotein. It is a nectin-like molecule belonging to Ig superfamily. CD155 is primarily found on endothelial cells, monocytes, epithelia and central nervous system. CD155 is an adhesion molecule involved in cell-cell and cell-matrix adhesion through interaction with CD226, CD96 (Tactile), nectin-1, -2, and -3 (CD111, CD112, CD113), and vitronectin. It also serves as a receptor for poliovirus and cytomegalovirus.
Poliovirus receptor (PVR), nectin-like 5
Type I glycoprotein, Ig superfamily
Monocytes, endothelial cells, epithelia, central nervous system
Cell-cell and cell-matrix adhesion
CD226, CD96, nectin-1, -2, and -3, vitronectin, CD155, poliovirus
1. Zola H, et al. eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication 2. Bottino PD, et al. 2005. Mol. Immunol. 42:463 3. Tahara-Hanaoka S, et al. 2004. Intl. Immunol. 16:533
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PE anti-human CD155 (PVR)
Human histiocytic lymphoma cells U-937 stained with SKII.4 PE
Purified anti-human CD155 (PVR)
Human histiocytic lymphoma cells U-937 stained with purified SKII.4, followed by anti-mouse IgG FITC