Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation4, and costimulation1,5 of IFN-gamma production and T cell proliferation. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 306310).
Application References:
1. Garcia, V., et al., 2001. J. Immunol. 167:5719. 2. Vincent, S., et al., 2002. J. Virol. 76:6121. 3. Cocks, B., et al., 1995. Nature 376:260 4. Sayos, J., et al., 2001. Blood 97:3867. 5. Aversa, G., et al., 1997. J. Immunol. 158:4036.
Human peripheral blood lymphocytes stained with purified A12, followed by biotinylated anti-mouse IgG and Sav-PE
Description:
CD150 is a 70-95 kD type I transmembrane glycoprotein also known as SLAM or IPO-3. It is a member of the Ig superfamily. It is expressed on a subset of T cells, B cells, dendritic cells, and endothelial cells. The expression of CD150 is upregulated upon activation. CD150 binds to itself as the ligand to be involved in B cell costimulation, proliferation, immunoglobulin production, and signal transduction.
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