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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kD carbohydrate structure, also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections.
Application References:
1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 3. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York. 4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood granulocytes stained with purified HI98, followed by anti-mouse IgGs FITC
CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.
Other Names:
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with HI98 APC
FITC anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained
with HI98 FITC
PE anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with HI98 PE
Purified anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with purified HI98, followed by anti-mouse IgGs FITC
Alexa Fluor® 488 anti-human CD15 (SSEA-1)
Human peripheral blood granulocytes stained with HI98 Alexa Fluor® 488
