Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections.
Application References:
1. Knapp, W., et al., Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 3. McMichael, A., et al., 1987. Leucocyte Typing III. Oxford University Press. New York.
Human peripheral blood granulocytes stained with purified HI98, followed by anti-mouse IgGs FITC
Description:
CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.
Other Names:
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Structure:
Poly-N-acetyllactosamine
Distribution:
Neutrophils, eosinophils, monocytes
Function:
Adhesion
Antigen References:
1. Stocks, S.C., et al., 1990. Biochem. J. 268:275.
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