The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C.
Application:
FC - Quality tested IHC(Frozen)*, IF5* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. McMichael, A., et al., 1987. Leucocyte Typing III. Oxford University Press. New York. 2. Knapp, W., et al., Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 3. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 4. Power, C. P., et al., 2004. J. Immunol. 173:5229. 5. Williams, K. C., et al., 2001. J. Exp. Med. 193:905. 6. Iwamoto, S., et al., 2007. J. Immunol. 179:1449.PubMed
Human peripheral blood monocytes stained
with M5E2 APC
Description:
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.
Other Names:
LPS receptor
Structure:
GPI-linked membrane glycoprotein, 53-55 kD
Distribution:
Monocytes, macrophages, granulocytes (low)
Function:
LPS receptor, clearance of Gram-negative pathogens
Ligand Receptor:
LPS
Antigen References:
1. Stocks, S., et al., 1990. Biochem. J. 268:275. 2. Wright, S., et al., 1990. Science 249:1434.
