Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: inhibition of tumor-cell invasion and blocking of aminopeptidase activities2,3, and immunohistochemical staining of acetone-fixed frozen tissue sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 301708).
Application References:
1. Knapp, W., et al., 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Saiki, I., et al., 1993. Int J Cancer. 54:137. 3. Rosenzwajg, M., et al., 2000. Blood 95:453. 4. Kawase, M., et al. 2008. J Virol.PubMed
Human peripheral blood granulocytes stained with purified WM15, followed by anti-mouse IgGs FITC
Description:
CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. This zinc metallopeptidase as a homodimer is expressed on granulocytes, myeloid progenitors, endothelial cells, epithelial cells and subset of granular lymphoid cells. It is not expressed on platelets or erythrocytes. CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the cleavage of chemokines such as MIP-1. CD13 serves as the cellular receptor for Coronavirus.
Other Names:
Aminopeptidase N, APN, gp150
Structure:
Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD
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