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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking1 of the binding of ICAM-1 and fibrinogen. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 301408).
Application References:
1. Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block) 2. Bryn T, et al. 2006. J. Immunol. 176:7361.J. Immunol. 3. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. 4. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed 5. Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed 6. Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed 7. Cavnar PJ, et al. 2011. J Cell Biol. 193:465. PubMed. 8. Theodoridis AA, et al. 2011. Blood. 118:107. PubMed. 9. Curran CS., et al. 2011. Int Immunol 23:713. PubMed.
PMA activated human granulocytes stained with CBRM1/5 FITC
The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils, as well as adhesion, phagocytosis and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and Factor X. The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the α chain. The CBRM1/5 antibody blocks cell adhesion to fibrinogen and ICAM-1.
Other Names:
Integrin αM chain, C3biR, CR3, Mac-1, Mo1
Structure:
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 KD
Distribution:
Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD11b (activated)
PMA activated human granulocytes stained with CBRM1/5 FITC
PE anti-human CD11b (activated)
PMA activated human granulocytes stained with CBRM1/5 FITC
Purified anti-human CD11b (activated)
PMA activated human granulocytes stained with CBRM1/5 FITC
APC anti-human CD11b (activated)
PMA-stimulated (5 minutes) human peripheral blood granulocytes stained with CBRM1/5 APC.
