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Purified Anti-human CD119 (IFN-γ R α Chain) Antibody
Material Safety Data Sheet (MSDS) Purified Anti-human CD119 IFN-gamma R Alpha Chain Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-human CD119 (IFN-γ R α Chain) Antibody
308702 100 µg $95.00     
Clone: GIR-94
Isotype: Mouse IgG2b, κ
Reactivity: Human
Immunogen: Human IFN-γRα, purified from human placenta
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application: FC-Quality Tested
WB3,4, IP3,4, IF6* - *This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications (for the relevant formats) include: Western blotting3,4, immunoprecipitation3,4, immunofluorescence microscopy6. GIR-94 antibody does not inhibit IFN-γ binding to IFN-γ R α chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308704) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308706) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application
References:
1. Sheehan, K., et al., 1988. J. Immunol. 140:4231.
2. Jouanguy, E., et al., 2000. J. Clin. Invest. 105:1429.
3. Kaplan, D., et al., 1996. J. Biol. Chem. 271:9.
4. Bach, E. A., et al., 1996. Mol. Cell. Biol. 16:3214.
5. Choi, J. C., et al., 2007. J. Immunol. 178:1598.
6. Humlicek, A.L., et al., 2007. J. Immunol. 178:6395.
Human peripheral blood lymphocytes stained

Human peripheral blood lymphocytes stained with purified GIR-94, followed by biotinylated anti-mouse IgGs and Sav-PE


Description: CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
Other Names: IFN-γR, IFN-γRα, IFN-α R1, CDw119
Structure: Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
Distribution: Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts
Function: Activates Jak1, Jak2, STAT1, host defense
Ligand Receptor: IFN-γ
Antigen
References:
1. Calderon, J., et al., 1988. PNAS 85:4837.
2. Basler, C., et al., 2002. Int. Rev. Immunol. 21:305.
3. Brierley, M., et al., 2002. J. Interferon Cytokine Res. 22:835.
AKP Goat anti-mouse IgG (minimal x-reactivity)Poly4053ELISA,WB
APC Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Biotin Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC, ELISA, IHC, IF, WB
HRP Goat anti-mouse IgG (minimal x-reactivity)Poly4053ELISA,IHC,WB
Purified Mouse IgG2b, κ Isotype CtrlMPC-11FC, ICC, IF, IHC, IP, WB
PE Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC,ICFC
Purified anti-human IFN-γ R β chain2HUB-159FC,IP,WB
Purified anti-human CD119 (IFN-γ R α chain)GIR-208FC,IHC,IP,WB
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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