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Purified Anti-human CD119 (IFN-γ R α Chain) Antibody
Material Safety Data Sheet (MSDS) Purified Anti-human CD119 IFN-gamma R Alpha Chain Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-human CD119 (IFN-γ R α Chain) Antibody
308601 This catalog number has been discontinued.
308602 100 µg $95.00     
Clone: GIR-208
Isotype: Mouse IgG1, κ
Workshop
Number:
VI C-110
Reactivity: Human
Immunogen: Human IFN-γRα, purified from human placenta
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application: FC - Quality tested
WB1*, IP1*, IHC(Frozen)3* - *This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is tested by immunofluorescence staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications (for the relevant formats) include: Western blotting1 under non-reducing conditions, immunoprecipitation1, immunohistochemistry3 of snap frozen sections, and blocking1 of IFN-γ binding to IFN-γ R α chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308604).
Application
References:
1. Sheehan, K., et al., 1988. J. Immunol. 140:4231.
2. Kishimoto, T., et al., Eds. 1997. Leucocyte Typing VI. Garland Publishing, Inc. London.
3. Peyman, J. A., et al., 1992. J. Immunol. 149:2675.
Human peripheral blood lymphocytes stained
with

Human peripheral blood lymphocytes stained with purified GIR-208, then detected with biotinylated anti-mouse IgG followed by Sav-PE


Description: CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
Other Names: IFN-γR, IFN-γRα, IFN-α R1, CDw119
Structure: Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
Distribution: Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts
Function: Activates Jak1, Jak2, STAT1, host defense
Ligand Receptor: IFN-γ
Antigen
References:
1. Calderon, J., et al., 1988. PNAS 85:4837.
2. Basler, C., et al., 2002. Int. Rev. Immunol. 21:305.
3. Brierley, M., et al., 2002. J. Interferon Cytokine Res. 22:835.
Purified Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICC, IF, IHC, IP, WB
Biotin Goat anti-mouse IgG (minimal x-reactivity)Poly4053ELISA,FC, WB
PE Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Streptavidin-PEStreptavidinFC,ICFC
Streptavidin-PE/Cy5StreptavidinFC,ICFC
Cell Staining Buffer (FBS)FC,ICC,ICFC
RBC Lysis Buffer (10X)FC,ICFC
Purified anti-human IFN-γ R β chain2HUB-159FC,IP,WB
Purified anti-human CD119 (IFN-γ R α chain)GIR-94FC,IF,IP,WB