Each lot of this antibody is tested by immunofluorescence staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting1 under non-reducing conditions, immunoprecipitation1, immunohistochemistry3 of snap frozen sections, and blocking1 of IFN-γ binding to IFN-γ R α chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308604).
Application References:
1. Sheehan, K., et al., 1988. J. Immunol. 140:4231. 2. Kishimoto, T., et al., Eds. 1997. Leucocyte Typing VI. Garland Publishing, Inc. London. 3. Peyman, J. A., et al., 1992. J. Immunol. 149:2675.
Human peripheral blood lymphocytes stained
with purified GIR-208, then
detected with biotinylated anti-mouse
IgG followed by Sav-PE
Description:
CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
Other Names:
IFN-γR, IFN-γRα, IFN-α R1, CDw119
Structure:
Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
Distribution:
Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts
Function:
Activates Jak1, Jak2, STAT1, host defense
Ligand Receptor:
IFN-γ
Antigen References:
1. Calderon, J., et al., 1988. PNAS 85:4837. 2. Basler, C., et al., 2002. Int. Rev. Immunol. 21:305. 3. Brierley, M., et al., 2002. J. Interferon Cytokine Res. 22:835.
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