Antibodies
Search 
 
Products Site Search
Advanced Search Login Cart
Home Company Products Web Tools Pathways Support Contact

Purified Anti-human CD119 (IFN-γ R α Chain) Antibody
Material Safety Data Sheet (MSDS) Purified Anti-human CD119 IFN-gamma R Alpha Chain Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-human CD119 (IFN-γ R α Chain) Antibody
308602 100 µg $95.00     
Clone: GIR-208
Isotype: Mouse IgG1, κ
Workshop
Number:
VI C-110
Reactivity: Human
Immunogen: Human IFN-γRα, purified from human placenta
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C.
Application: FC - Quality tested
WB1*, IP1*, IHC(Frozen)3* - *This application has been reported in the literature.
Recommended Usage: Each lot of this antibody is tested by immunofluorescence staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications (for the relevant formats) include: Western blotting1 under non-reducing conditions, immunoprecipitation1, immunohistochemistry3 of snap frozen sections, and blocking1 of IFN-γ binding to IFN-γ R α chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308604).
Application
References:
1. Sheehan, K., et al., 1988. J. Immunol. 140:4231.
2. Kishimoto, T., et al., Eds. 1997. Leucocyte Typing VI. Garland Publishing, Inc. London.
3. Peyman, J. A., et al., 1992. J. Immunol. 149:2675.
Human peripheral blood lymphocytes stained
with

Human peripheral blood lymphocytes stained with purified GIR-208, then detected with biotinylated anti-mouse IgG followed by Sav-PE


Description: CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
Other Names: IFN-γR, IFN-γRα, IFN-α R1, CDw119
Structure: Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
Distribution: Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts
Function: Activates Jak1, Jak2, STAT1, host defense
Ligand Receptor: IFN-γ
Antigen
References:
1. Calderon, J., et al., 1988. PNAS 85:4837.
2. Basler, C., et al., 2002. Int. Rev. Immunol. 21:305.
3. Brierley, M., et al., 2002. J. Interferon Cytokine Res. 22:835.
Purified Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICC, IF, IHC, IP, WB
Biotin Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC, ELISA, IHC, IF, WB
PE Goat anti-mouse IgG (minimal x-reactivity)Poly4053FC
Streptavidin-PEStreptavidinFC,ICFC
Streptavidin-PE/Cy5StreptavidinFC,ICFC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC,ICFC
Purified anti-human IFN-γ R β chain2HUB-159FC,IP,WB
Purified anti-human CD119 (IFN-γ R α chain)GIR-94FC,IF,IP,WB
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
BioLegend - Home | Company |  Products | Pathways |  Support | Ordering |  Careers | Contact |  Distributors | Sitemap |     
Copyright © 2010 BioLegend, Inc. All rights reserved.