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Purified anti-human CD119 (IFN-γ R α chain) Antibody
Purified anti-human CD119 (IFN-γ R α chain) Antibody
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting1 under non-reducing conditions, immunoprecipitation1, immunohistochemistry3 of snap frozen sections, and blocking1 of IFN-γ binding to IFN-γ R α chain. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 308604).
Application References:
1. Sheehan K, et al. 1988. J. Immunol. 140:4231. (WB IP Block) 2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. 3. Peyman JA, et al. 1992. J. Immunol. 149:2675. (IHC)
Human peripheral blood lymphocytes stained
with purified GIR-208, then
detected with biotinylated anti-mouse
IgG followed by Sav-PE
CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
Other Names:
IFN-γR, IFN-γRα, IFN-α R1, CDw119
Structure:
Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
Distribution:
Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts
Function:
Activates Jak1, Jak2, STAT1, host defense
Ligand Receptor:
IFN-γ
Antigen References:
1. Calderon J, et al. 1988. P. Natl. Acad. Sci. USA 85:4837. 2. Basler C, et al. 2002. Int. Rev. Immunol. 21:305. 3. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
LEAF™ Purified anti-human CD119 (IFN-γ R α chain)
Human peripheral blood lymphocytes stained with LEAF™ purified GIR-208, detected with biotinylated anti-mouse IgGs and Sav-PE
Purified anti-human CD119 (IFN-γ R α chain)
Human peripheral blood lymphocytes stained
with purified GIR-208, then
detected with biotinylated anti-mouse
IgG followed by Sav-PE
PE anti-human CD119 (IFN-γ R α chain)
Human peripheral blood lymphocytes stained
with purified GIR-208, then
detected with biotinylated anti-mouse
IgG followed by Sav-PE
