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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Layton JE, et al. 1997. Growth Factors. 14:117 2. Layton JE, et al. 1999. J. Biol. Chem. 274:17445 3. Layton JE, et al. 2001. J. Biol. Chem. 276:36779
Human peripheral blood granulocytes stained with LMM741 PE
CD114 is the receptor of the colony stimulating factor 3 (CSF3, G-CSF), consist of two 130 kD, type I transmembrane chains that form a homodimer. The extracellular domain consists of an immunoglobulin-like domain, a cytokine receptor homologue domain, and three fibronectin type III repeats. CD114 is expressed in all stages of granulocyte differentiation and in monocytes, platelets, endothelial cells, placenta and trophoblasts. The binding of CSF3, results in the activation of many signaling molecules such as Syk, Lyn, Jak1, Jak2, Tyk2, SOCS3, SOCS1, STAT5, and Shp1, resulting in the expression of different target genes that will increase neutrophil precursor survival, proliferation and maturation. In mature neutrophils, it increases survival, superoxide anion generation, arachidonic acid release, production of myeloperoxidase and leukocyte alkaline phosphatase (LAP).
Single chain type I transmembrane molecule of 130 kD. The extracellular domain consists of an immunoglobulin-like domain, a cytokine receptor homologue domain, and three fibronectin type III repeats. To function as a receptor, this molecules form a homod
Distribution:
Granulocytes (in all stages of differentiation), monocytes, platelets, endothelial cells, placenta and trophoblasts.
Function:
Stimulates survival, proliferation, and maturation of neutrophil precursors. In mature neutrophils increases survival, superoxide anion generation, arachidonic acid release, production of myeloperoxidase and leukocyte alkaline phosphatase (LAP).
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD114 (G-CSFR)
Human peripheral blood granulocytes stained with LMM741 PE
PE anti-human CD114 (G-CSFR)
Human peripheral blood granulocytes stained with LMM741 PE
APC anti-human CD114 (G-CSFR)
Human peripheral blood granulocytes from stained with LMM741 APC
