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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Human peripheral blood granulocytes stained with purified 1D9-M12 conjugated to PE
C5L2 (C5a receptor-like 2) is a seven-transmembrane orphan receptor, also known as GPR77, which shares significant homology to C5a receptor (CD88). But it is a decoy receptor without G-protein coupling. C5L2 is co-expressed with C5aR on many kinds of cells, such as neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes. C5L2 binds C5a with similar high affinity as C5aR and relatively higher affinity for C5a-des-Arg than with C5aR. Clone 1D9-M12 is known to block C5a binding to C5L2, but does not react with C5a receptor.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human C5L2
Human peripheral blood granulocytes stained with purified 1D9-M12 conjugated to PE
