Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional reported applications (for the relevant formats) include: Western blotting, and ELISA.
β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.
β2M, β2-M, beta2-microglobulin
Nonpolymorphic Ig like structure, noncovalently associated with heavy chain of MHC class I molecules, 12 kD
Govern MHC class I molecule stability, promote MHC class I molecules binding and presenting peptide antigens to CD8+ T cells
CD3/TCR complex, CD8
1. Engelhard VH. 1994. Curr. Opin. Immunol. 6:13. 2. Williams DB, et al. 1989. J. Immunol. 142:2796. 3. Danliczyk UG and TL. Delovitch. 1994. J. Immunol. 153:3533. 4. Williams A, et al. 2002. Tissue Antigens 59:3.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with purified 2M2, followed by anti-mouse FITC
FITC anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with 2M2 FITC
PE anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with 2M2 PE
Biotin anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with biotinylated 2M2, followed by Sav-PE
APC anti-human β2-microglobulin
Human peripheral blood lymphocytes stained with 2M2 APC