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Purified anti-human β2-microglobulin Antibody
Cat. # Size Price  

316302 100 µg $95.00
    
Product Details

Clone: 2M2 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Purified Mouse IgG1, κ Isotype Ctrl
Reactivity: Human, Cross-Reactivity*: Swine (Pig, Porcine)
Immunogen: Purified human β2-microglobulin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
WB, ELISA - Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Additional reported applications (for the relevant formats) include: Western blotting, and ELISA.
Application
References:

  Publication Library
 Image 1
Human peripheral blood lymphocytes stained with purified 2M2, followed by anti-mouse FITC


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Antigen Details

Description: β2-microglobulin (β2M) is a 12 kD nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kD polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, β2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells. β2M plays an essential role both in governing MHC class I molecules stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.
Other Names: β2M, β2-M, beta2-microglobulin
Structure: Nonpolymorphic Ig like structure, noncovalently associated with heavy chain of MHC class I molecules, 12 kD
Distribution: Leukocytes, platelets, endothelial cells, epithelial cells
Function: Govern MHC class I molecule stability, promote MHC class I molecules binding and presenting peptide antigens to CD8+ T cells
Ligand Receptor: CD3/TCR complex, CD8
Antigen
References:
1. Engelhard VH. 1994. Curr. Opin. Immunol. 6:13.
2. Williams DB, et al. 1989. J. Immunol. 142:2796.
3. Danliczyk UG and TL. Delovitch. 1994. J. Immunol. 153:3533.
4. Williams A, et al. 2002. Tissue Antigens 59:3.
GeneID: 567
UniProt: View information about beta2-microglobulin on UniProt.org
Keywords: Purified anti-human β2-microglobulin, 2M2, Purified, β2M, β2-M, beta2-microglobulin, Human, Cross-Reactivity*: Swine (Pig, Porcine), Flow Cytometry, ELISA, Western Blotting, Immunology, Antibodies
Technical Data Sheet (pdf)
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Related Protocols

Cell Surface Immunofluorescence Staining Protocol

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human β2-microglobulin
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified 2M2, followed by anti-mouse FITC

  • FITC anti-human β2-microglobulin
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 2M2 FITC

  • PE anti-human β2-microglobulin
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 2M2 PE

  • Biotin anti-human β2-microglobulin
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated 2M2, followed by Sav-PE

  • APC anti-human β2-microglobulin
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with 2M2 APC

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