Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by activated monocytes/macrophages and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.
CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
Ig superfamily, B7 family, 40 kD
B cells and dendritic cells
Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
1. Wang S, et al. 2002. J. Biol. Chem. 96:2808. 2. Wong SC, et al. 2003. Blood 102:1831.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human CD275 (B7-H2, ICOSL)
Human monocyte-derived dendritic cells stained with 2D3 PE
Purified anti-human CD275 (B7-H2, ICOSL)
Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE
Biotin anti-human CD275 (B7-H2, ICOSL)
Human monocyte-derived dendritic cells stained with biotinylated 2D3,followed by Sav-PE
APC anti-human CD275 (B7-H2, ICOSL)
Human Burkitt's lymphoma cell line Daudi was stained with CD275 (clone 2D3) APC (filled histogram) or Mouse IgG2b, κ APC isotype control (open histogram).