Antibodies
      
 
Product Search  
 
Purified anti-human CD275 (B7-H2, ICOSL) Antibody
Cat. # Size Price  

309402 100 µg $95.00
    
Product Details

Clone: 2D3 (See other available formats)
Isotype: Mouse IgG2b, κ
Isotype Control:Purified Mouse IgG2b, κ Isotype Ctrl
Reactivity: Human
Immunogen: Human B7H2-mIg fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
References:
  Publication Library
 Image 1
Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE


Compare all formats





 


Antigen Details

Description: B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by activated monocytes/macrophages and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.
Other Names: CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
Structure: Ig superfamily, B7 family, 40 kD
Distribution: B cells and dendritic cells
Function: Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
Ligand Receptor: ICOS
Antigen
References:
1. Wang S, et al. 2002. J. Biol. Chem. 96:2808.
2. Wong SC, et al. 2003. Blood 102:1831.
GeneID: 23308
UniProt: View information about CD275 on UniProt.org
Keywords: Purified anti-human CD275 (B7-H2, ICOSL), 2D3, Purified, CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1, Human, Flow Cytometry, Immunoprecipitation, ELISA, Immunology, Antibodies
Technical Data Sheet (pdf)
Material Safety Data Sheet
Certificate of Analysis
Request Bulk Quote
Request Custom Conjugation
Email Technical Support
Write a Review
View All CD275 Reagents
Related Protocols

Cell Surface Immunofluorescence Staining Protocol
Rating

This product has not been reviewed yet.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2013-07-08
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human CD275 (B7-H2, ICOSL)
    Human monocyte-derived dendritic cells stained

    Human monocyte-derived dendritic cells stained with 2D3 PE

  • Purified anti-human CD275 (B7-H2, ICOSL)
    Human monocyte-derived dendritic cells stained

    Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE

  • Biotin anti-human CD275 (B7-H2, ICOSL)
    Human monocyte-derived dendritic cells stained

    Human monocyte-derived dendritic cells stained with biotinylated 2D3,followed by Sav-PE

  • APC anti-human CD275 (B7-H2, ICOSL)
    Human Burkitt's lymphoma cell line

    Human Burkitt's lymphoma cell line Daudi was stained with CD275 (clone 2D3) APC (filled histogram) or Mouse IgG2b, κ APC isotype control (open histogram).

  • PE/Cy7 anti-human CD275 (B7-H2, ICOSL)
    Human Burkitt’s lymphoma cell line,

    Human Burkitt’s lymphoma cell line, Daudi was stained with CD275 (clone 2D3) PE/Cy7 (filled histogram), or mouse IgG2b, κ isotype PE/Cy7 (open histogram).

x
x
*Your Rating:
*Your Comment:
*Name (first, last):
*Affiliation:
*Email:
*Required fields
Note: Submission of a quick review does not qualify for our promotional $25 Amazon gift card. A full review submission is required for gift card eligibility.

Learn more…
9727 Pacific Heights Blvd.
San Diego, CA 92121

Tel: 858.768.5800
Company
Careers
Promotions
Web Tools
Areas of Biology
Pathways
Products
Literature
Custom Products/Services
Custom Panel Construction
Technical Support
Support Resources
Ordering
Contact
Distributors
Sitemap
Connect with us:

Facebook Twitter Pinterest LinkedIn YouTube Tumblr Blog Podcast
The LEGENDeers Pandora Google+ Wikipedia RSS Feed Arcade Words With Elements Satisfaction Survey
Privacy Policy | Terms
World-Class Quality | Superior Customer Support | Outstanding Value Copyright © 2014 BioLegend, Inc. All rights reserved.
Product Search