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Purified anti-human CD275 (B7-H2, ICOSL) Antibody
Purified anti-human CD275 (B7-H2, ICOSL) Antibody
309402 100 µg $95.00 Qty:
    

Product Details

 
Clone: 2D3 (See other available formats)
Isotype: Mouse IgG2b, κ
Isotype Control:Purified Mouse IgG2b, κ Isotype Ctrl
Reactivity: Human
Immunogen: Human B7H2-mIg fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Application
References:

  Publication Library
Purified anti-human CD275 (B7-H2, ICOSL) Antibody 2D3 Image 1
Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE


Compare all formats



Cell Surface Immunofluorescence Staining Protocol




Description:

B7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by activated monocytes/macrophages and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.

Other Names: CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1
Structure: Ig superfamily, B7 family, 40 kD
Distribution: B cells and dendritic cells
Function: Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production
Ligand Receptor: ICOS
Antigen
References:

1. Wang S, et al. 2002. J. Biol. Chem. 96:2808.
2. Wong SC, et al. 2003. Blood 102:1831.

GeneID: 23308
Latest Publications: View the latest CD275 articles on HighwirePress.com
UniProt: View information about CD275 on UniProt.org
Keywords: Purified anti-human CD275 (B7-H2, ICOSL), 2D3, Purified, CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1, Human, Flow Cytometry, Immunoprecipitation, ELISA, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
Biotin anti-human CD275 (B7-H2, ICOSL)2D3FC
PE anti-human CD275 (B7-H2, ICOSL)2D3FC


Related Pathways

Pathway
Dendritic Cells
ICos-ICosL Pathway in T-Helper Cell
Immunologic Networks 2011
T Follicular Helper Cell Pathway


Related Categories

Category
2D3
Immunology > Human Immunology > CD and Related Molecules > CD275 (ICOSL, B7H2, B7-H2) > 2D3
2D3
Immunology > Human Immunology > CD and Related Molecules > B7-H2 (ICOSL, B7H2, CD275) > 2D3
2D3
Immunology > Human Immunology > Dendritic cells > CD275(B7-H2, ICOSL)

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Version: 2 Revision Date: 2013-07-08
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human CD275 (B7-H2, ICOSL)
    Human monocyte-derived dendritic cells stained

    Human monocyte-derived dendritic cells stained with 2D3 PE

  • Purified anti-human CD275 (B7-H2, ICOSL)
    Human monocyte-derived dendritic cells stained

    Human monocyte-derived dendritic cells stained with purified 2D3, then detected with biotinylated anti-mouse IgGs and Sav-PE

  • Biotin anti-human CD275 (B7-H2, ICOSL)
    Human monocyte-derived dendritic cells stained

    Human monocyte-derived dendritic cells stained with biotinylated 2D3,followed by Sav-PE

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