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Human, Mouse *This antibody also reacts to GST derived from Schistosoma Japonicum. It has been confirmed that it does not bind endogenous human and mouse GST.
Immunogen:
GST- N-terminal HRF fusion protein
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by affinity chromatography.
Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.2 - 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
HeLa (Lane 1) and A20 (Lane2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with polyclonal anti-HRF/N19 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and chemiluminescence detection.
HRF (histamine-releasing factor), also known as TCTP, TPT1, p02, and fortilin, is a member of the TCTP family of calcium-binding proteins. It is widely expressed and apparently serves as a transcriptional activator, calcium transporter, histamine inducer, and antiapoptotic, caspase-3 inhibitor. HRF, in addition to functioning as a histamine-releasing factor, can modulate secretion of cytokines from human basophils, eosinophils, T cells, B cells, and epithelial cells. HRF knockout mice were embryonic lethal. Transgenic mice models have shown that HRF augments the inflammatory response to allergens.
Other Names:
p02, TCTP, FLJ27337, TPT1
Structure:
HRF
Distribution:
Cytoplasm and secreted
Function:
Involved in calcium binding and microtubule stabilization.
Ligand Receptor:
Human HRF is 172 amino acids (aa) in length, with multiple potential splice variants. HRF is a highly conserved protein. Its aa sequence is 96% identical to mouse HRF.
Interaction:
Interacts with STEAP3
Antigen References:
1. Sanchez JC, et al. 1997. Electrophoresis 18:150. 2. Yoon T, et al. 2001. Arch. Biochem. Biophys. 384:379. 3. Bommer UA, et al. 2002. RNA 8:478. 4. MacDonald SM, et al. 1995. Science 269:688. 5. Yeh YC, et al. 2010. PLoS One 5:e11077.
Purified anti-HRF, Poly6165, Purified, p02, TCTP, FLJ27337, TPT1, Human, Mouse *This antibody also reacts to GST derived from Schistosoma Japonicum. It has been confirmed that it does not bind endogenous human and mouse GST., Western Blotting, Immunology, Antibodies
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Purified anti-HRF
HeLa (Lane 1) and A20 (Lane2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with polyclonal anti-HRF/N19 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and chemiluminescence detection.
