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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
hnRNP K (Heterogeneous nuclear ribonucleoprotein K) is a pre-mRNA binding protein and a component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. This protein containing KH domains and is ubiquitously expressed in the cytoplasm and the nucleus with a predicted molecular weight approximately 51 kD. hnRNP K expression and function are increased by epidermal growth factor (EGF). hnRNP K acts as transcriptional regulator and functions in nucleocytoplasmic shuttling, and is modified by phosphorylation (PKC δ, c-Src) and acetylation. Clone F45P9C7 has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat, rabbit and chicken hnRNP K.
Ubiquitously expressed cytoplasmic and nuclear protein
Function:
Pre-mRNA binding protein, component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. Acts as transcriptional regulator and functions in nucleocytoplasmic shuttling
Regulation:
Epidermal growth factor (EGF) can increase expression and function
Interacts with Csk, NCK2, Vav1, ITK, CEBP β, c-Src, Fyn, Lyn, Hepatitis virus core protein
Antigen References:
1. Matunis MJ, et al. 1992. Mol. Cell. Biol. 12:164. 2. Dejgaard K, et al. 1994. J. Mol. Biol. 236:33. 3. Mandal M, et al. 2001. J. Biol. Chem. 276:9699
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Purified anti-hnRNPK
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-hnRNPK antibody (clone F45P9C7). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
