This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Extracts from Hela cells treated overnight with 10 mM nocodazole were resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit antibody against methylated hnRNP K (Lys219). Lane 1, cells in complete medium alone; Lane 2, nocodazole-treated cells. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Description:
hnRNP K (also known as heterogeneous nuclear ribonucleoprotein K, DC-stretch binding protein, and transformation upregulated protein (TUNP)) is a 51 kD heterogenous nuclear ribonucleoprotein family containing KH domains. This protein is ubiquitously expressed in the cytoplasm and the nucleus. hnRNP K is a pre-mRNA binding protein and a component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. hnRNP K acts as transcriptional regulator and functions in nucleocytoplasmic shuttling. hnRNP K expression and function are increased by epidermal growth factor (EGF). hnRNP K is modified by phosphorylation (PKC δ, c-Src) and acetylation. hnRNP K interacts with Csk, NCK2, Vav1, ITK, CEBP β, c-Src, Fyn, Lyn, and Hepatitis virus core protein. The Poly6253 antibody recognizes human methylated hnRNP K (Lys219) and has been shown to be useful for Western blotting.
Ubiquitously expressed cytoplasmic and nuclear protein
Function:
Pre-mRNA binding protein, component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. Acts as transcriptional regulator and functions in nucleocytoplasmic shuttling
Regulation:
Epidermal growth factor (EGF) can increase expression and function
Interacts with Csk, NCK2, Vav1, ITK, CEBP β, c-Src, Fyn, Lyn, Hepatitis virus core protein
Antigen References:
1. Matunis, M. J., et al., 1992. Mol. Cell. Biol. 12:164. 2. Dejgaard, K., et al., 1994. J. Mol. Biol. 236:33. 3. Mandal, M., et al., 2001. J. Biol. Chem. 276:9699
