Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Takada, Y., et al. 2011. Development 138:4207. PubMed.
Hela cells were fixed in 4% paraformaldehyde , washed in PBS, and permeabilized using 0.5% Triton X-100 for 5 minutes prior to antibody staining. Cells were incubated with a 1:50 dilution of Histone H4 antibody (Poly6020) for 1 hr, washed twice using 0.1% Triton X-100 in PBS and incubated with goat anti-rabbit conjugated to Alexa Fluor® 488. After washing twice in 0.1% Triton X-100 in PBS, cells were mounted and examined on a fluorescent scope. 100 X magnification.
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Histone H4 antibody (clone Poly6020). Proteins were visualized using a HRP-conjugated anti-rabbit-IgG secondary and chemiluminescence detection
Histone H4 is an 11.4 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. Histone H4 shows transcriptional regulation that is both cell cycle-dependent and -independent. Histone H4 is modified by phosphorylation, acetylation, ribosylation, and methylation. Histone H4 has been shown to interact with other histone acetytransferases, histone deacetylases, and PARP. The Poly6020 antibody has been shown to be useful for the detection of human histone H4 by Western blot and immunofluorescence staining.
Structure:
11.4 kD
Distribution:
Nuclear
Function:
Component of octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). Creates nucleosome structure of chromosomal fiber in eukaryotes; dynamic gene regulation
Regulation:
Transcriptional regulation both cell cycle-dependent and -independent
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Histone H4
Hela cells were fixed in 4% paraformaldehyde , washed in PBS, and permeabilized using 0.5% Triton X-100 for 5 minutes prior to antibody staining. Cells were incubated with a 1:50 dilution of Histone H4 antibody (Poly6020) for 1 hr, washed twice using 0.1% Triton X-100 in PBS and incubated with goat anti-rabbit conjugated to Alexa Fluor® 488. After washing twice in 0.1% Triton X-100 in PBS, cells were mounted and examined on a fluorescent scope. 100 X magnification.
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Histone H4 antibody (clone Poly6020). Proteins were visualized using a HRP-conjugated anti-rabbit-IgG secondary and chemiluminescence detection
