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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Hirata A, et al. 2004. J. Histochem. Cytochem. 52:1503. 2. Goto H, et al. 1999. J. Biol. Chem. 274:25543. 3. Ozawa K. 2008. Cytometry A 73:517.
Untreated HeLa (Panel A) and overnight nocodazoled-treated HeLa (Panel B) were stained with purified rat monoclonal antibody against phospho-H3 (Ser28) (clone HTA28), followed by Alexa Fluor® 488 anti-alpha-tubulin, DyLight™ 594 goat anti-rat-IgG and DAPI.
Western blot analysis of extracts from untreated Hela cells (lane 1) or overnight nocodazole-treated Hela cells (lane 2), using anti-phospho-Histone H3 (Ser28), clone HTA28.
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.
Other Names:
H3
Structure:
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution:
Nucleus
Function:
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Regulation:
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Interaction:
Two molecules of H3 form a heterotetramer with two molecules of H4.
Antigen References:
1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545. 2. Goto H, et al. 2002. Genes Cells 7:11. 3. Garcia BA, et al. 2005. Biochemistry 44:13202.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Histone H3-Phosphorylated (Ser28)
Untreated HeLa (Panel A) and overnight nocodazoled-treated HeLa (Panel B) were stained with purified rat monoclonal antibody against phospho-H3 (Ser28) (clone HTA28), followed by Alexa Fluor® 488 anti-alpha-tubulin, DyLight™ 594 goat anti-rat-IgG and DAPI.
Western blot analysis of extracts from untreated Hela cells (lane 1) or overnight nocodazole-treated Hela cells (lane 2), using anti-phospho-Histone H3 (Ser28), clone HTA28.
