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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.
293T cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-H3-pS10 clone 11D8 antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1, is inter phase 293T cell extract; lane 2, is M phase 293T cell extract (overnight nocodazole-treated cells).
Exponentially growing Hela cells were stained with purified phospor-Histone H3 (Ser10), clone 11D8 antibody, followed by Alexa Fluor® 488 conjugated to anti-mouse IgG and DAPI. The phosphor-Histone staining is specific on metaphase cells only.
Histone H3 is phosphorylated at serine 10 during mitosis and found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.
Other Names:
H3
Structure:
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution:
Nucleus
Function:
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
Regulation:
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Interaction:
Two molecules of H3 form a heterotetramer with two molecules of H4.
Antigen References:
1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545. 2. Goto H, et al. 2002. Genes Cells 7:11. 3. Garcia BA, et al. 2005. Biochemistry 44:13202. 4. Hans F, et al. 2001. Oncogene 20:3021.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Histone H3 Phospho (Ser10)
293T cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-H3-pS10 clone 11D8 antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1, is inter phase 293T cell extract; lane 2, is M phase 293T cell extract (overnight nocodazole-treated cells).
Exponentially growing Hela cells were stained with purified phospor-Histone H3 (Ser10), clone 11D8 antibody, followed by Alexa Fluor® 488 conjugated to anti-mouse IgG and DAPI. The phosphor-Histone staining is specific on metaphase cells only.
Alexa Fluor® 488 anti-Histone H3 Phospho (Ser10)
Hela cells were treated with 20 µM of Nocodazole for 24 hours then fixed, permeabilized with 70% ethanol, and then stained with anti-Histone H3 Phospho-Ser10 (clone 11D8) Alexa Flour® 488 or mouse IgG2b, κ Alexa Flour® 488 isotype control.
