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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Fixed and permeabilized Hela cells were stained with a 1:50 dilution of HDAC4 antibody (Poly6077) for 1 hr, incubated with goat anti-rabbit conjugated to Alexa Fluor® 488 (top panel). Cells were examined at 100 X magnification. DAPI stained cells are shown as a control (bottom panel).
NIH 3T3 (left lane) and Jurkat (right lane) nuclear extracts were probed with rabbit anti-HDAC4 antibody by Western Blot.
HDAC4 is a 140 kD class II histone deacetylase similar to the yeast HDA1 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC4 uses active nuclear export to shuttle between the nucleus and the cytoplasm. HDAC4 is modified by phosphorylation by CaM kinase I or IV. HDAC4 has been reported to interact with 14-3-3 in the cytoplasm and MEF2A, HDAC3, NcoR and SMRT in the nucleus. The Poly6077 antibody has been shown to be useful for the detection of human (Western blotting and immunofluorescence) and mouse (Western blotting; immunofluorescence not tested) HDAC4 protein.
Other Names:
Histone deacetylase 4
Structure:
Class II histone deacetylase family, highly homologous to yeast HDA1; 140 kD
Distribution:
Uses active nuclear export to shuttle between nucleus and cytoplasm
Function:
Operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues
Regulation:
HDAC activity has been associated with transcriptional repression, nucleosomal condensation
Modification:
Phosphorylation by CaM kinase I or IV, S
Interaction:
Associates with 14-3-3 in cytoplasm and MEF2A in nucleus, interacts with HDAC3, NcoR, SMART
Antigen References:
1. Tong JK, et al. 1998. Nature 395:917. 2. Grunstein M. 1997. Nature 389:349. 3. Wolffe A. 1996. Science 272:371. 4. Lee DY, et al. 1993. Cell 72:73. 5. Wang A, et al. 2001. Mol. Cell. Biol. 21:5992. 6. Ertos M, et al. 1999. Mol. Cell. Biol. 19:7816.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-HDAC4
Fixed and permeabilized Hela cells were stained with a 1:50 dilution of HDAC4 antibody (Poly6077) for 1 hr, incubated with goat anti-rabbit conjugated to Alexa Fluor® 488 (top panel). Cells were examined at 100 X magnification. DAPI stained cells are shown as a control (bottom panel).
NIH 3T3 (left lane) and Jurkat (right lane) nuclear extracts were probed with rabbit anti-HDAC4 antibody by Western Blot.
