This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB, IF
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-HDAC3. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Immunofluorescent microscope analysis of Hela cells using anti-HDAC3 polyclonal antibody (poly6067) (red). α-tubulin (clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue).
Description:
HDAC3 is a 50 kD class I histone deacetylase similar to yeast RPD3 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC3 is modified by phosphorylation and sumoylation. HDAC3 has been reported to interact with HDAC4, SMRT and N-CoR co-repressors, GATA-2 and TF-II-I. The Poly6076 antibody has been shown to be useful for Western blotting and immunofluorescence detection of human HDAC3.
Other Names:
Histone deacetylase 3
Structure:
Class I histone deacetylase family, similar to yeast RPD3 protein; 50 kD
Distribution:
Nuclear
Function:
Operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues
Regulation:
HDAC activity has been associated with transcriptional repression, nucleosomal condensation
Modification:
Phosphorylation, Sumoylation
Interaction:
Interacts with HDAC4, SMRT and N-CoR co-repressors, GATA-2, and TF-II-I
Antigen References:
1. Tong, J., et al., 1998. Nature 395:917. 2. Grunstein, M. 1997. Nature 389:349. 3. Wolffe, A., 1996. Science 272:371. 4. Lee, D., et al., 1993. Cell 72:73.
