Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Beels L, et al. 2009. Circulation. PubMed 2. Ramirez-Herrick AM, et al. 2011. Blood 117:2681. PubMed 3. Li M, et al. 2011. Mol Cell Biol. PubMed 4. Boyd, MT., et al. 2011. J. Cell Biol. 5:689. PubMed.
Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-H2A.X antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Hela cells were fixed in 4% paraformaldehyde , washed in PBS, and permeabilized using 0.5% Triton X-100 for 5 minutes. Cells were then incubated with a 1:50 dilution of H2A.X antibody (Poly6133) for 1 hr, washed twice using 0.1% Triton X-100 in PBS and incubated with goat anti-rabbit conjugated to Alexa Fluor® 488. After washing twice, cells were mounted and examined on a fluorescence microscope. 100 X magnification.
H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The Poly6133 antibody has been shown to be useful for Western blotting and immunofluorescence of human H2A.X.
Other Names:
H2A.x, H2a/x, Histone 2A, Histone 2A.X
Structure:
Basal histone, member of the H2 histone family; 14 kD
Distribution:
Nuclear
Function:
Phosphorylated H2AX promotes DNA repair and maintains genomic stability. Important for recombination between immunoglobulin switch regions
Regulation:
Synthesized in G1 and S-phase of cell cycle
Modification:
Phosphorylation on Ser139 after double-stranded DNA breaks
Antigen References:
1. Mannironi C, et al.1989. Nucleic Acids Res. 17:9113. 2. Celeste A, et al. 2002. Science 296:922. 3. Bassing CH, et al. 2002. P. Natl. Acad. Sci. USA 99:8173. 4. Reina-San-Martin B, et al. 2003. J. Exp. Med. 197:1767.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-H2A.X
Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-H2A.X antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Hela cells were fixed in 4% paraformaldehyde , washed in PBS, and permeabilized using 0.5% Triton X-100 for 5 minutes. Cells were then incubated with a 1:50 dilution of H2A.X antibody (Poly6133) for 1 hr, washed twice using 0.1% Triton X-100 in PBS and incubated with goat anti-rabbit conjugated to Alexa Fluor® 488. After washing twice, cells were mounted and examined on a fluorescence microscope. 100 X magnification.
