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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-GSK 3ß antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Recombinant human full length GSK3 beat containing an N-terminal GST tag protein (400 ng per lane) was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-GSK 3ß antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
GSK-3β (glycogen synthase kinase-3 beta) is a 49 kD serine/threonine kinase that is a member of the CDC2/CDKX subfamily. This protein is expressed in the testis, thymus, prostate, and ovary, with weak expression in lung, brain, and kidney. GSK-3β is thought to participate in the insulin and Wnt signaling pathway and has been shown to phosphorylate c-jun to reduce DNA binding. GSK-3β can be modified by AKT phosphorylation which inhibits function. This protein has been shown to interact with axin and GBP/FRAT. The Poly6042 antibody recognizes human and mouse GSK-3β and is cross-reactive with GSK-3α. The Poly6042 antibody has been shown to be useful for Western blotting.
Other Names:
Glycogen synthase kinase-3 beta
Structure:
Serine/Threonine family protein kinases, CDC2/CDKX subfamily, monomer; 49 kD
Distribution:
Testis, thymus, prostate, ovary. Weak expression in lung, brain, kidney
Function:
Participates in insulin and Wnt signaling pathway, phosphorylates c-jun to reduce DNA binding
Regulation:
Inhibited by AKT phosphorylation
Modification:
Phosphorylation
Interaction:
Interacts with axin, GBP/FRAT
Antigen References:
1. Stambolic V, et al. 1994. Biochem. J. 303:701. 2. Delcommenne M, et al. 1998. P. Natl. Acad. Sci. USA 95:11211.
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Purified anti-GSK-3β
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-GSK 3ß antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Recombinant human full length GSK3 beat containing an N-terminal GST tag protein (400 ng per lane) was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-GSK 3ß antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
