This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB - Quality Tested
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 to 2.0 µg/ml antibody dilution for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
HepG2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with purified rabbit polyclonal anti-Gli-1 antibody, proteins were visualized by HRP conjugated anti-rabbit secondary antibody and a chemiluminescence system.
Description:
This gene encodes a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined; however, it may play a role in normal development gene transcription. The Gli transcription factors constitute the final effectors of the Hedgehog signalling pathway, which is critical to vertebrate development. Mouse mutation studies indicate possible involvement in human foregut malformation. In addition, both the Ras and Transforming Growth Factor Beta pathways also stimulate and/or induce Gli1 and Gli2 activity independently of Hedgehog ligands.
Other Names:
Glioma-associated oncogene homolog 1
Structure:
Zinc-finger-containing transcription factors
Distribution:
Nuclear and cytosol
Function:
Mainly regulated by the Hedgehog pathway.
Interaction:
Regulate the transcription of Pax2, Sal1, N-myc, CyclinD, Bmp4 and other genes by binding to their Gli consensus sequence.
Antigen References:
1. Riddle et al., Cell 1993; 75:1417 2. Fernández-Zapico, et al., Pancreatology. 2008;8(3):227
